Molecular comparison of bacterial isolates from blood with strains colonizing pharynx and intestine in immunocompromised patients with sepsis
Molecular comparison of bacterial isolates from blood with strains colonizing pharynx and intestine in immunocompromised patients with sepsis
复制标题
DOI：
10.1099/jmm.0.05076-0
复制
发表时间：
2003-06-01
影响因子：
3
通讯作者：
Fujieda, K
中科院分区：
文献类型：
--
作者：
Murono, K;Hirano, Y;Fujieda, K
免疫功能低下患者的脓毒症的大多数致病微生物与那些定植于自己鼻咽或肠道的细菌是同一物种。为了确定从血液中分离到的菌株是否主要来自患者自身的菌群，对从血液和咽喉和/或粪便中分离的菌株进行了基因组分析。对接受强化化疗和造血干细胞移植治疗的癌症患者的咽喉和粪便进行前瞻性监测培养。在从血液中分离的菌株与从咽喉和/或粪便中分离的菌株属于同一物种的脓毒症病例中，用PFGE比较这些菌株的基因组图谱。在两年的时间里，在14名受试者中有6人记录了10例血培养阳性败血症；分离出铜绿假单胞菌、表皮葡萄球菌、肠球菌、绿色链球菌和梭杆菌。都是从血液中找到的。在7例血液分离株与咽喉或粪便分离株同种的7例中，有5例两地分离株的基因型别完全相同。在大多数免疫功能低下的患者中，血液感染的致病微生物主要来自他们自己的菌群。
Most causative organisms of sepsis in immunocompromised patients are the same species as those that colonize their own nasopharynx or intestinal tract. To determine whether the strains recovered from blood originate mainly from patients' own flora, isolates from blood and throat and/or stool were investigated by genomic analyses. Surveillance cultures of throat and stool were taken prospectively from cancer patients being treated with intensive chemotherapy followed by haematopoietic stem-cell transplantation. In those cases of sepsis in which the isolate from blood was the same species as that from the throat and/or stool, the genomic profiles of the isolates were compared by PFGE. Ten cases of blood culture-positive sepsis were documented in six of 14 subjects during a 2 year period; isolates of Pseudomonas aeruginosa, Staphylococcus epidermidis, Enterococcus sp., viridans streptococci and Fusobacterium sp. were recovered from blood. In five of seven cases in which the blood isolate was the same species as that from the throat or stool, the genotypes of the isolates from both sites were identical. In the majority of immunocompromised patients, the causative organisms of bloodstream infections originated mainly from their own flora.