Structural characterization and cell response evaluation of electrospun PCL membranes: Micrometric versus submicrometric fibers
Structural characterization and cell response evaluation of electrospun PCL membranes: Micrometric versus submicrometric fibers
复制标题
DOI：
10.1002/jbm.a.32048
复制
发表时间：
2009-06-15
影响因子：
4.9
通讯作者：
Grigioni, Mauro
中科院分区：
文献类型：
--
作者：
Del Gaudio, Costantino;Bianco, Alessandra;Grigioni, Mauro
静电纺丝技术是一种制备组织工程纤维支架的有效方法。典型的非织造结构允许细胞粘附和增殖，并支持营养物和废物的扩散。聚（F-己内酯）（PCL）电纺膜从（a）四氢呋喃和N，N-二甲基甲酰胺的1：1混合物和（B）氯仿中的14%w/v溶液开始制备。得到由无珠的随机排列的均匀纤维组成的基质。平均纤维直径为（a）0.8 ± 0.2 μ m和（B）3.6 ± 0.8 μ m。PCL基质显示出以下拉伸机械性能：拉伸模量（a）5.0 +/-0.7 MPa（B）6.4 +/-0.2 MPa，屈服应力（a）0.55 +/-0.06 MPa（B）0.43 +/-0.02 MPa，以及极限拉伸应力（a）1.7 +/-0.2 MPa和（B）0.8 +/-0.1 MPa。极限应变范围在300%和400%之间。采用细胞-基质电阻抗传感技术，以人脐静脉内皮细胞（HUVEC）为实验材料，对静电纺膜的细胞毒性进行了连续检测。PCL基质不含有毒量的污染物和/或工艺副产物。通过在微米和亚微米纤维垫上培养HUVEC进行的体外研究表明，这两种结构都支持细胞粘附和扩散。然而，在微米网络上培养的细胞显示出更高的活力和与聚合物纤维的改善的相互作用，这表明促进细胞定殖的能力增加。(c)2008 Wiley Periodicals，Inc. J Biomed Mater Res 89A：1028 - 1039，2009
Electrospinning is a valuable technique to fabricate fibrous scaffolds for tissue engineering. The typical nonwoven architecture allows cell adhesion and proliferation, and supports diffusion of nutrients and waste products. Poly(F-caprolactone) (PCL) electrospun membranes were produced starting from 14% w/v solutions in (a) mixture 1:1 tetrahydrofuran and N,N-dimethylformamide and (b) chloroform. Matrices made up of randomly arranged uniform fibers free of beads were obtained. The average fiber diameters were (a) 0.8 +/- 0.2 mu m and (b) 3.6 +/- 0.8 pm. PCL matrices showed the following tensile mechanical properties: tensile modulus (a) 5.0 +/- 0.7 MPa (b) 6.4 +/- 0.2 MPa, yield stress (a) 0.55 +/- 0.06 MPa (b) 0.43 +/- 0.02 MPa, and ultimate tensile stress (a) 1.7 +/- 0.2 MPa and (b) 0.8 +/- 0.1 MPa. The ultimate strain ranged between 300% and 400%. Cytotoxicity of electrospun membranes was continuously evaluated by means of electric cell-substrate impedance sensing technique using human umbilical vein endothelial cells (HUVEC). PCL matrices resulted free of toxic amounts of contaminants and/or process by-products. In vitro studies performed by culturing HUVEC on micrometric and submicrometric fibrous mats showed that both structures supported cell adhesion and spreading. However, cells cultured on the micrometric network showed higher vitality and improved interaction with the polymeric fibers, suggesting an increased ability to promote cell colonization. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 89A: 1028-1039, 2009