Enhanced IgA class switching in marginal zone and B1B cells relative to follicular/B2 B cells
Enhanced IgA class switching in marginal zone and B1B cells relative to follicular/B2 B cells
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DOI：
10.4049/jimmunol.177.9.6025
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发表时间：
2006-11-01
影响因子：
4.4
通讯作者：
Stavnezer, Janet
中科院分区：
文献类型：
--
作者：
Kaminski, Denise A.;Stavnezer, Janet
与滤泡（FO）/B2 B细胞相比，在腹腔中富集的小鼠脾边缘区（MZ）B细胞和B1 B细胞优先响应T细胞非依赖性Ag。尽管B细胞亚群对各种刺激的反应不同，尽管需要多种刺激来诱导伊加类别转换，但B细胞亚群对伊加产生的相对贡献是未知的。通过培养纯化的B细胞群，我们发现在BLyS/LPS/TGF-β中，MZ和腹膜B1细胞比脾FO或腹膜B2细胞更容易转换为伊加。向培养物中添加IL-4、IL-5和抗IgD葡聚糖可将FO/B2和MZ B细胞中的伊加转换增强至相似的频率，但这种处理抑制B1细胞中的伊加类别转换。因此，伊加转换在纯化的B细胞亚群中不同，这表明个体B细胞群体可以根据可用的刺激对体内伊加表达做出不同的贡献。
Mouse splenic marginal zone (MZ) B cells and B1 B cells enriched in the peritoneal cavity respond preferentially to T cell-independent Ags compared with follicular (FO)/B2 B cells. Despite the differential responses of B cell subsets to various stimuli, and despite the need for multiple stimuli to induce IgA class switching, the relative contribution of B cell subpopulations to IgA production is unknown. By culturing purified B cell populations, we find that MZ and peritoneal B1 cells switch more readily to IgA than do splenic FO or peritoneal B2 cells in BLyS/LPS/TGF-beta. Addition of IL-4, IL-5, and anti-IgD dextran to the cultures enhances IgA switching in FO/B2 and MZ B cells to a similar frequency, but this treatment suppresses IgA class switching in B1 cells. Thus, IgA switching differs among purified B cell subsets, suggesting that individual B cell populations could contribute differentially to IgA expression in vivo, depending on available stimuli.