Improved survival of injured sciatic nerve Schwann cells in mice lacking the p75 receptor
Improved survival of injured sciatic nerve Schwann cells in mice lacking the p75 receptor
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DOI：
10.1016/s0304-3940(99)00618-7
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发表时间：
1999-09-17
影响因子：
2.5
通讯作者：
Bisby, MA
中科院分区：
文献类型：
--
作者：
Ferri, CC;Bisby, MA
神经损伤后，雪旺细胞上p75神经营养因子受体的表达显著增加（Heumann， R., Korsching， S., bandflow, C ., and Thoenen， H.，坐骨神经横断后非神经元细胞中神经生长因子合成的变化）。[j]细胞生物学。， 104(1987) 1623-1631。Taniuchi， M., Clark, h.b., Schweitzer, j.b., and Johnson, e.m.，神经生长因子在周围神经剪断雪旺细胞中的表达：超微结构定位，轴突接触抑制和结合特性。j . >。， 8(1988) 664-681.)，然而p75受体在损伤后的作用尚不清楚。先前的研究表明，p75受体可能在几种细胞类型的凋亡中起作用。为了更好地了解p75受体在神经损伤后事件中的作用，我们比较了缺乏功能p75受体的成年小鼠和Balb-C（野生型）小鼠受损坐骨神经的凋亡情况。坐骨神经挤压或切除损伤后，我们在5天、21天和4个月后用荧光FragEL DNA片段法检测远端神经节段细胞凋亡程度。两种小鼠的神经损伤均诱导了大量的凋亡细胞核，但p75基因敲除小鼠的凋亡细胞密度在损伤后21天低于Balb-C小鼠。p75受体可促进雪旺细胞在轴突向失神经残端再生过程中的凋亡。(C) 1999出版，Elsevier Science ireland Ltd.版权所有。
Following nerve injury, there is a dramatic increase in the expression of the p75 neurotrophin receptor on Schwann cells (Heumann, R., Korsching, S., Bandtlow, C, and Thoenen, H., Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection. J. Cell Biol., 104 (1987) 1623-1631. Taniuchi, M., Clark, H.B., Schweitzer, J.B, and Johnson, E.M., Expression of nerve growth factor by Schwann cells of axotomized peripheral nerves: ultrastructural location, suppression by axonal contact, and binding properties. J. Neurosci., 8 (1988) 664-681.), however the role of the p75 receptor following injury remains unclear. Previous studies have shown that the p75 receptor may play a role in the apoptosis of several cell types. To better understand the role of the p75 receptor in the events following nerve injury, we have compared apoptosis in injured sciatic nerves of adult mice lacking functional p75 receptors and Balb-C (wild-type) mice. Following sciatic nerve crush or resection injuries, we used a fluorescent FragEL DNA fragmentation method to examine the extent of cellular apoptosis in distal nerve segments 5 days, 21 days and 4 months later. Nerve injury induced large numbers of apoptotic nuclei in nerves of both strains, but in p75 knockout mice, the density of apoptotic cells was lower compared to Balb-C mice, 21 days following injury. The p75 receptor may promote apoptosis in Schwann cells when axons are regenerating into the denervated nerve stump. (C) 1999 Published by Elsevier Science lreland Ltd. All rights reserved.