Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery.
Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery.
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DOI：
10.1364/boe.411648
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发表时间：
2021-02-01
影响因子：
3.4
通讯作者：
Notingher I
中科院分区：
文献类型：
--
作者：
Lizio MG;Liao Z;Shipp DW;Boitor R;Mihai R;Sharp JS;Russell M;Khout H;Rakha EA;Notingher I
乳腺癌的标准治疗是主要通过保乳手术（BCS）进行手术切除。我们开发了一种基于自体荧光（AF）光谱成像和拉曼光谱的新技术，用于快速术中评估BCS的切除边缘。将基于全内反射（TIR）的新型宽视场AF成像单元与配备有785 nm激光器的拉曼光谱显微镜相结合。AF激发的波长被优化为365 nm，以最大化脂肪组织的区分。该方法允许通过拉曼光谱测量更有效地靶向组织的非脂肪区域，所述非脂肪区域具有较高的包含肿瘤的风险。集成的TIR-AF-拉曼在小组织样本以及新鲜的广泛局部切除上进行了测试，在不到45分钟的时间内对BCS标本（5.1 × 7.6 cm 2）的整个交叉表面进行了分析，并提供了有关标本中肿瘤位置的信息。仪器的完全自动化和选择更快的平移台将允许在术中时间尺度（20分钟）内测量BCS标本。这项研究表明，TIR-AF拉曼显微镜代表了一个可行的步骤，在术中时间尺度内的大WLE的术中评估技术的发展。
The standard treatment for breast cancer is surgical removal mainly through breast-conserving surgery (BCS). We developed a new technique based on auto-fluorescence (AF) spectral imaging and Raman spectroscopy for fast intraoperative assessment of excision margins in BCS. A new wide-field AF imaging unit based on total internal reflection (TIR) was combined with a Raman spectroscopy microscope equipped with a 785 nm laser. The wavelength of the AF excitation was optimized to 365 nm in order to maximize the discrimination of adipose tissue. This approach allows for the non-adipose regions of tissue, which are at a higher risk of containing a tumor, to be targeted more efficiently by the Raman spectroscopy measurements. The integrated TIR-AF-Raman was tested on small tissue samples as well as fresh wide local excisions, delivering the analysis of the entire cruciate surface of BCS specimens (5.1 × 7.6 cm2) in less than 45 minutes and also providing information regarding the location of the tumor in the specimen. Full automation of the instrument and selection of a faster translation stage would allow for the measurement of BCS specimens within an intraoperative time scale (20 minutes). This study demonstrates that the TIR-AF Raman microscope represents a feasible step towards the development of a technique for intraoperative assessment of large WLE within intraoperative timescales.