A preliminary account of the application of thin-sectioning, freeze-etching, and scanning electron microscopy to the study of coralline algae
A preliminary account of the application of thin-sectioning, freeze-etching, and scanning electron microscopy to the study of coralline algae
复制标题
薄切片、冷冻蚀刻和扫描电子显微镜在珊瑚藻研究中的应用初述
DOI：
10.2216/i0031-8884-9-1-83.1
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发表时间：
1970
期刊：
影响因子：
1.6
通讯作者：
T. Bisalputra
中科院分区：
文献类型：
--
作者：
A. Bailey;T. Bisalputra
摘要对南美白绒蒿Cordiformia vagulveriensis Yendo和南美白绒蒿C. officinalis L.表明只有表面和紧邻的亚表面细胞是完整的，顶端的大多数细胞具有退化的原生质体。扫描显微镜观察表明，顶端节的表面是网状的，每个空间的网有一个中心孔。薄切片表明，孔是，事实上，一个残坑连接在内部切向壁的侵蚀表面细胞，并存在这种结构接近顶点表明，叶状体表面的侵蚀是迅速的。顶端的薄切片也显示高尔基体系统发育良好，产生许多大的小泡，尽管其他大的小泡可能是由内质网的退化造成的。通过冷冻蚀刻新鲜和固定的材料制备的复制品显示，大囊泡直接与Cordella和Calliarthron的石灰质壁区域融合，一些在壁基质内保持完整。O...
Abstract Preliminary studies of Corallina vancouveriensis Yendo and C. officinalis L. suggest that only the surface and immediately subsurface cells are intact, the majority of the cells at the apex having degenerate protoplasts. Scanning microscopy shows that the surface of the apical segment is reticulate, each space of the reticulum having a central pore. Thin sections indicate that the pore is, in fact, a relict pit connection in the inner tangential wall of the eroded surface cells, and the presence of this structure close to the apex suggests that erosion of the thallus surface is rapid. Thin sections at the apex also show that Golgi systems are well developed, producing numerous large vesicles, though other large vesicles may possibly result from degeneration of the endoplasimc reticulum. Replicas prepared by freeze-etching of fresh and fixed material show that large vesicles fuse directly with the calcareous wall region in Corallina and Calliarthron, some remaining intact within the wall matrix. O...