Detoxified lethal toxin as a potential mucosal vaccine against anthrax.
Detoxified lethal toxin as a potential mucosal vaccine against anthrax.
复制标题
解毒的致命毒素作为潜在的炭疽粘膜疫苗。
DOI：
10.1128/cvi.00402-07
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Zeng,Mingtao
中科院分区：
文献类型：
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作者：
Xu,Qingfu;Zeng,Mingtao
The nontoxic mutant lethal factor (mLF; which has the E687C substitution) and functional protective antigen (PA63) ofBacillus anthraciswere evaluated for their use as mucosal vaccines against anthrax in A/J mice. Intranasal vaccination of three doses of 30 μg of mLF or 60 μg of PA63 elicited significant serum and mucosal antibody responses, with anthrax lethal toxin-neutralizing titers of 40 and 60 in immune sera, respectively. However, only 30% and 60% of the vaccinated animals in the two groups could survive a challenge with 100 times the 50% lethal dose ofB. anthracisSterne spores, respectively. In contrast, vaccination with three doses of the combination of 30 μg of mLF and 60 μg of PA63, the detoxified lethal toxin, elicited antibody responses against LF and PA significantly higher than those elicited after vaccination with mLF or PA63 individually by use of the same dose and schedule. Vaccination with the detoxified lethal toxin resulted in significantly higher lethal toxin-neutralizing antibody titers in sera (titer, 90). Animals vaccinated with three doses of the detoxified lethal toxin were completely protected against the spore challenge. The data suggest that mLF and PA63 have a mutual enhancement effect for evoking systemic and mucosal immune responses and that the detoxified lethal toxin can be used as an efficient mucosal vaccine against anthrax.
