Simultaneous determination of all aminobutyric acids by chiral derivatization and liquid chromatography-tandem mass spectrometry
Simultaneous determination of all aminobutyric acids by chiral derivatization and liquid chromatography-tandem mass spectrometry
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手性衍生化液相色谱-串联质谱法同时测定所有氨基丁酸
DOI：
10.1007/s44211-023-00293-w
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发表时间：
2023
影响因子：
1.6
通讯作者：
Todoroki Kenichiro
中科院分区：
文献类型：
--
作者：
Sugiyama Eiji;Nakamura Mao;Mizuno Hajime;Furusho Aogu;Todoroki Kenichiro
氨基丁酸包括八种结构或立体异构体，其表现出广泛的生物活性。最近的证据表明，一些低丰度异构体增加了对所有异构体的高选择性分析的需求;然而，所有氨基丁酸异构体的同时分离还没有成功，除了一种专门的方法，使用多个分离柱和样品的分裂。本研究建立了一种新的分析方法，利用手性衍生化和液相色谱-串联质谱在一个单一的分离柱中分离所有的氨基丁酸异构体。所有的非对映体衍生物在C18柱中被解析，并且衍生物在串联质谱中显示出特征性的裂解模式。利用该方法，我们分析了拟南芥种子中的异构体，发现存在三种低丰度异构体，d-、l-β-氨基异丁酸和d-β-氨基丁酸。该方法使用市售的手性衍生试剂和广泛使用的色谱柱，因此可广泛应用于生物和食品分析。
Aminobutyric acids include eight structural or stereoisomers that exhibit a wide range of biological activities. Recent evidence on some low abundant isomers have increased the demand for highly selective analysis of all the isomers; however, simultaneous separation of all the aminobutyric acid isomers has not been successful yet, except for a specialized method that uses multiple separation columns and a split of samples. In this study, we developed a new analytical method using chiral derivatization and liquid chromatography-tandem mass spectrometry to separate all the aminobutyric acid isomers in a single separation column. All the diastereomeric derivatives were resolved in a C18 column, and the derivatives showed characteristic fragmentation patterns in tandem mass spectrometry. By using the method, we analyzed the isomers in theArabidopsis thalianaseeds and revealed the existence of three low abundant isomers, i.e.,d-,l-β-aminoisobutyric acid, andd-β-aminobutyric acid. The proposed method uses a commercially available chiral derivatizing reagent and a broadly used column; therefore, it can be widely used in biological and food analyses.Graphical abstract