A genome-wide transcriptional analysis of the mitotic cell cycle
A genome-wide transcriptional analysis of the mitotic cell cycle
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DOI：
10.1016/s1097-2765(00)80114-8
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发表时间：
1998-07-01
期刊：
影响因子：
16
通讯作者：
Davis, RW
中科院分区：
文献类型：
--
作者：
Cho, RJ;Campbell, MJ;Davis, RW
已知通过真核细胞周期的进展既受调节，又伴随着许多基因表达水平的周期性波动。我们在这里报告了在发芽酵母菌酿酒酵母的细胞周期中mRNA转录水平的全基因组表征。在6220个受监测的转录本中，发现416个细胞周期依赖性周期性。在基因组中直接发现了416个基因中超过25％的基因，这些基因在同一细胞周期阶段表现出诱导，这表明在全球mRNA调节中局部染色体组织的机制。超过60％的表现出mRNA波动的特征基因已经与细胞周期特异性生物学作用有关。由于超过20％的人蛋白与酵母蛋白表现出重要同源性，因此这些结果还将一系列人类基因与细胞周期周期特异性生物学功能联系起来。
Progression through the eukaryotic cell cycle is known to be both regulated and accompanied by periodic fluctuation in the expression levels of numerous genes. We report here the genome-wide characterization of mRNA transcript levels during the cell cycle of the budding yeast S. cerevisiae. Cell cycle-dependent periodicity was found for 416 of the 6220 monitored transcripts. More than 25% of the 416 genes were found directly adjacent to other genes in the genome that displayed induction in the same cell cycle phase, suggesting a mechanism for local chromosomal organization in global mRNA regulation. More than 60% of the characterized genes that displayed mRNA fluctuation have already been implicated in cell cycle period-specific biological roles. Because more than 20% of human proteins display significant homology to yeast proteins, these results also link a range of human genes to cell cycle period-specific biological functions.