Localization of various secretory phospholipase A2 enzymes in male reproductive organs
Localization of various secretory phospholipase A2 enzymes in male reproductive organs
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DOI：
10.1016/j.bbalip.2004.08.017
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发表时间：
2004-11-08
影响因子：
4.8
通讯作者：
Kudo, I
中科院分区：
文献类型：
--
作者：
Masuda, S;Murakami, M;Kudo, I
目前的证据表明，在男性生殖器官中存在几种分泌型磷脂酶A(2)(Spla(2))的转录本。本研究用免疫组织化学方法检测了IIA、IIC、IID、IIE、IIF、V和X Spla组蛋白在男性生殖器官中的定位。在SplA(2)-IIA缺陷的C57BL/6小鼠睾丸中，Spla(2)-IIC、-IID、-IIE、-IIF、-V和-X在生精小管生精细胞中有不同程度的表达。免疫印迹显示这些Spla(2)S存在于小鼠精子中。此外，Spla(2)-IIF、-V和-X定位于间质细胞。在小鼠培养的间质细胞系中也检测到了同样的sPLA2，腺病毒介导的这些sPLA2 S S转入间质细胞后，前列腺素的产生增加。Spla(2)-IIC、-IID、-IIE、-IIF、-V和-X在附睾腺、输精管、精囊和前列腺的上皮中也有不同程度的表达。在一株SplA(2)-IIA阳性的FVB株中，在间质细胞中检测到SplA(2)-IIA的弱表达。Spla(2)的表达谱在小鼠和人类的精囊和前列腺中发现了显著的差异。综上所述，个体Spla(2)S在男性生殖器官中表现出明显或部分重叠的定位，暗示了特定和多余的功能。(C)2004爱思唯尔B.V.保留所有权利。
Current evidence suggests the presence of transcripts for several secretory phospholipase A(2) (sPLA(2)) enzymes in male genital organs. In this study, we examined by immunohistochemistry the localization of group IIA, IIC, IID, IIE, IIF, V and X sPLA(2)s in male genital organs. In sPLA(2)-IIA-deficient C57BL/6 mouse testis, sPLA(2)-IIC, -IID, -IIE, -IIF, -V and -X were diversely expressed in spermatogenic cells within the seminiferous tubules. Immunoblotting revealed the presence of these sPLA(2)s in mouse spermatozoa. In addition, sPLA(2)-IIF, -V and -X were localized in the interstitial Leydig cells. The same set of sPLA2S was detected in a mouse cultured Leydig cell line, and adenovirus-mediated transfer of these sPLA(2)s into Leydig cells resulted in increased prostaglandin production. sPLA(2)-IIC, -IID, -IIE, -IIF, -V and -X were also detected diversely in the epithelium of the epididymis, vas deferens, seminal vesicles, and prostate. In a sPLA(2)-IIA-positive FVB strain, weak expression of sPLA(2)-IIA was detected in Leydig cells. Notable differences in the sPLA(2) expression profiles were found in the seminal vesicles and prostate between mice and humans. Taken together, individual sPLA(2)s exhibit distinct or partially overlapping localizations in male reproductive organs, suggesting both specific and redundant functions. (C) 2004 Elsevier B.V. All rights reserved.