Cell-to-cell communication within intact human skin.
Cell-to-cell communication within intact human skin.
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完整人体皮肤内的细胞间通讯。
DOI：
10.1172/jci113578
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Meda,P
中科院分区：
文献类型：
--
作者：
Salomon,D;Saurat,JH;Meda,P
我们的特点是细胞与细胞通信（耦合）在完整的人皮肤显微注射单个角质形成细胞与缝隙连接渗透示踪剂（荧光黄）。在大多数注射后，观察到来自表皮不同层的25-50个角质形成细胞偶联（n = 31）。还显微注射了一些非通讯细胞（n = 3），或在偶联的角质形成细胞的大片区域内观察到。真皮成纤维细胞的显微注射证明了广泛的偶联（大于100个成纤维细胞）;然而，角质形成细胞（n = 34）或成纤维细胞（n = 3）注射均未显示表皮和真皮隔室之间的偶联。细胞耦合被认为是更广泛的表皮脊比在suprapapillary板，在这两个地区，是不那么广泛的表皮基底层注射后，比后的suprabasal层。这项研究表明，连接细胞与细胞的通信发生在正常和完全分化的人体组织。收集的定量数据还表明完整成人皮肤内角质形成细胞与角质形成细胞通讯的区域异质性，以及类维生素A对这种模式缺乏影响。图片
We have characterized cell-to-cell communication (coupling) within intact human skin by microinjecting single keratinocytes with a gap junction-permeant tracer (Lucifer Yellow). 25-50 keratinocytes from different layers of the epidermis were seen to be coupled after most injections (n = 31). A few noncommunicating cells were also microinjected (n = 3) or observed within large territories of coupled keratinocytes. Microinjections of dermal fibroblasts demonstrated an extensive coupling (greater than 100 fibroblasts); however, none of the keratinocyte (n = 34) or fibroblast (n = 3) injections revealed coupling between the epidermal and dermal compartments. Cell coupling was found to be more extensive in epidermal ridges than in suprapapillary plates and, in both regions, was less extensive after injection of the basal layer of the epidermis than after that of the suprabasal layers. This study shows that junctional cell-to-cell communications take place in normal and fully differentiated human tissue. The quantitative data gathered also indicate a regional heterogeneity of keratinocyte-to-keratinocyte communication within intact adult skin and the lack of effect of retinoids on this pattern.Images