Use of animal models for the imaging and quantification of angiogenesis.
Use of animal models for the imaging and quantification of angiogenesis.
复制标题
使用动物模型进行血管生成的成像和定量
DOI：
10.1538/expanim.17-0054
复制
发表时间：
2018-02-09
影响因子：
2.4
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Liu M;Xie S;Zhou J
血管生成是从原始血管网络发育出新血管的过程；它是正常生理过程所必需的，例如胚胎发育和伤口愈合。血管生成也参与病理事件，包括心肌缺血和肿瘤生长。为了研究这一重要过程的分子机制，采用了多种方法和模型。这些策略还可用于深入了解血管生成相关疾病的病因学，从而有助于开发新的诊断和治疗方法。常用的动物模型包括鸡胚胎的绒毛尿囊膜和卵黄囊膜、小鼠视网膜和主动脉环以及植入小鼠体内的血管生成反应器。这些动物模型在血管生成过程的研究中发挥了重要作用。例如，绒毛尿囊膜在鸡胚胎发育过程中会经历强大的血管生成，并且由于其表面易于接近，因此该膜为实验提供了方便的模型。在这里，我们讨论采用动物模型进行血管生成成像和定量的方法。此外，我们还为该领域的未来研究提出了潜在的新方向。
Angiogenesis is the process of developing new blood vessels from the original vascular network; it is necessary for normal physiological processes, such as embryonic development and wound healing. Angiogenesis is also involved in pathological events, including myocardial ischemia and tumor growth. To investigate the molecular mechanisms of this important process, a variety of methods and models are employed. These strategies can also be used to provide insight into the etiology of angiogenesis-related diseases, thereby contributing to the development of new diagnostics and treatments. Commonly used animal models include the chorioallantoic membrane and yolk sac membrane of chick embryos, the mouse retina and aortic ring, and angiogenesis reactors implanted into mice. These animal models have been instrumental in the study of the angiogenic process. For example, the chorioallantoic membrane undergoes robust angiogenesis during the development of chick embryos, and, because its surface is easily accessible, this membrane provides a convenient model for experimentation. Here, we discuss the methods that employ animal models for the imaging and quantification of angiogenesis. In addition, we propose potential novel directions for future investigations in this area.