Effect of the Vrn1‐Fr1 Interval on Cold Hardiness Levels in Near‐Isogenic Wheat Lines
Effect of the Vrn1‐Fr1 Interval on Cold Hardiness Levels in Near‐Isogenic Wheat Lines
复制标题
Vrn1-Fr1间隔对近等基因小麦品系抗寒水平的影响
DOI：
10.2135/cropsci1998.0011183x003800020035x
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发表时间：
1998
期刊：
影响因子：
2.3
通讯作者：
M. Walker
中科院分区：
文献类型：
--
作者：
E. Storlie;R. Allan;M. Walker
利用位于5AL染色体上的Vrn1-Fr1区间，研究了小麦抗寒性调控的可行性。该区间含有一个影响春化要求和抗寒性水平的基因(S)。我们对Vrn1-Fr1区间等位基因不同的小麦近等基因系(近等基因系)进行了LT50试验(50%的植株致死温度)，以确定区间对耐寒小麦和非抗寒小麦抗寒性水平的影响。近等基因系是春小麦轮回亲本‘Mared’与两个冬小麦供体亲本‘Suweon 185’和‘Chugoku 81’的5个回交组合，它们分别是耐寒和非耐寒的。发达种群为苏维恩185/6*大田和楚国81/6*大田春、冬两季近等基因系。结果表明，冬季NILs比春季NILs能耐受4.3℃的低温LT50，苏威185/6*露地冬季NILs能耐受0.5℃的LT50，比中部81/6*露地冬季NILs低0.5℃。结果表明，Vrn1-Fr1间期可以解释LT50的71-91%的变异。用Xwg644探针对近等位基因进行分析，以确认其与Vrn1-Fr1区间的连锁，并确定其作为春化需求和抗寒性标记的实用性。用Xwg644对冬小麦和春小麦的DNA进行EcoRI酶切，发现一条9.7kb的条带与冬小麦的生长习性共分离。结果表明，冬小麦品种间的抗寒性差异可以用这一区间来解释，可以通过植物育种来调节这一区间，以提高品种的抗寒性。
We investigated the feasibility of cold hardiness manipulation in wheat, Triticum aestivum L., with the Vrn1-Fr1 interval on chromosome 5AL. The interval contains a gene(s) that has influenced vernalization requirements and cold hardiness levels. We conducted LT 50 tests (lethal temperature of 50% of plants) on wheat NILs (near-isogenic lines) that differed for alleles at the Vrn1-Fr1 interval, to determine the effects of the interval on cold hardiness levels between hardy and nonhardy wheat. The NILs were derived from five backcrosses between a spring wheat recurrent parent, 'Marfed', and two winter wheat donor parents, 'Suweon 185' and 'Chugoku 81', hardy and nonhardy, respectively. The developed populations were Suweon 185/6*Marfed and Chugoku 81/6*Marfed spring and winter NILs. Results showed that the winter NILs could tolerate a 4.3 °C colder LT 50 score than the spring NILs, and that the Suweon 185/6*Marfed winter NILs could tolerate a 0.5 °C colder LT 50 than Chugoku 81/ 6*Marfed winter NILs. Results indicate that the Vrn1-Fr1 interval explained between 71 and 91% of the variation for LT 50 scores between these genotypes. The NILs were analyzed with the probe Xwg644 to confirm linkage with the Vrn1-Fr1 interval and to determine its utility as a marker for vernalization requirement and cold hardiness. EcoRI-digested DNA of the winter and spring NIL wheat, probed with Xwg644, showed that a 9.7-kb band co-segregated with the winter growth habit. Results indicate that differences in cold hardiness levels between winter wheat cultivars may be explained by this interval and that the interval may be manipulated through plant breeding to improve the cold hardiness of cultivars.