LYMPHOCYTE ACTIVATING FACTOR PROMOTES T-CELL GROWTH-FACTOR PRODUCTION BY CLONED MURINE LYMPHOMA-CELLS
LYMPHOCYTE ACTIVATING FACTOR PROMOTES T-CELL GROWTH-FACTOR PRODUCTION BY CLONED MURINE LYMPHOMA-CELLS
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DOI：
10.1038/287853a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
FAVATA, MF
中科院分区：
文献类型：
--
作者：
SMITH, KA;GILBRIDE, KJ;FAVATA, MF
两种可溶性因子对于T细胞对抗原或凝集素的增殖应答是必需的。一种这样的因子，被认为是T细胞来源的，其特征在于其启动和维持连续T细胞生长的能力（T细胞生长因子，TCGF）。巨噬细胞来源的第二种活性增强凝集素引发的胸腺细胞或粘附细胞耗尽的脾细胞的增殖反应（淋巴细胞活化因子，LAF）。虽然有人认为LAF通过促进T细胞产生TCGF来促进T细胞增殖，但缺乏支持这一假设的直接证据。研究了一种小鼠胸腺淋巴瘤，其响应于凝集素而释放TCGF。在无血清、成分确定的培养基中，高度纯化的LAF促进TCGF释放的浓度依赖性增加。由于T细胞的增殖取决于可用的TCGF的浓度，这些结果表明，LAF和TCGF构成了一个基本的双峰扩增系统，其最终决定了T细胞克隆扩增的程度。
Two soluble factors are essential for a T-cell proliferative response to antigen or lectin. One such factor, thought to be of T-cell origin, is distinguished by its ability to initiate and maintain continuous T-cell growth (T-cell growth factor, TCGF). A 2nd activity of macrophage origin augments lectin-initiated proliferative responses of thymocytes or adherent cell-depleted splenocytes (lymphocyte-activating factor, LAF). Although it was suggested that LAF promotes T-cell proliferation by facilitating the production of TCGF by T-cells, direct evidence supporting this hypothesis was lacking. A murine thymic lymphoma which releases TCGF in response to lectin was studied. In serum-free, defined medium, highly purified LAF promotes a concentration-dependent increase in TCGF release. Because the proliferation of T cells depends on the concentration of TCGF available, these results indicate that LAF and TCGF constitute an essential bimodal amplification system which ultimately determines the extent of T-cell clonal expansion.