EFFECTS OF THALLIUM ON PRIMARY CULTURES OF TESTICULAR CELLS
EFFECTS OF THALLIUM ON PRIMARY CULTURES OF TESTICULAR CELLS
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DOI：
10.1080/15287399209531623
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发表时间：
1992-05-01
期刊：
影响因子：
--
通讯作者：
FAUSTMAN, EM
中科院分区：
文献类型：
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作者：
GREGOTTI, C;DINUCCI, A;FAUSTMAN, EM
这项体外研究的目的是检查支持细胞和生殖细胞混合培养物对铊（Tl）处理的反应，在以前的研究中，铊（Tl）的浓度范围在体内被证明会影响生殖。从Sprague-Dawley大鼠的睾丸中制备培养物。根据培养物的蛋白质含量估计，用对应于35、7和1.4 μ g Tl/g睾丸的Tl浓度处理含有约3.75 x 10(6)个细胞/ml的培养物。在处理后24、48和72 h的观察显示，生殖细胞显著释放到培养基中，这与浓度和时间有关。用34 μ g /g Tl/g睾丸进行培养，暴露24小时后，生殖细胞脱离的数量比对照组增加了三倍。随着处理时间增加到48 h，即使在最低的Tl浓度（1.4 μ g Tl/g睾丸）下，培养物也出现了明显的生殖细胞损失。48小时后，暴露于7 μ g Tl/g睾丸的培养物的生殖细胞脱离率比对照组增加了2.5倍，暴露于35 μ g Tl/g睾丸的培养物的生殖细胞脱离率比对照组增加了10倍。细胞培养的形态学研究显示生殖细胞明显缺失，前期和后期精母细胞显著减少，支持细胞形状发生变化。这些结果与体内研究一致，在体内研究中，在相当暴露水平的铊治疗中，最早表现出对睾丸支持细胞和生殖细胞的毒性作用。他们还证明了这种体外培养技术对快速评估毒性睾丸损伤的有用性。
The objective of this in vitro study was to examine the response of mixed cultures of Sertoli and germ cells to treatment with thallium (Tl) at the range of concentrations that, in previous studies, was shown in vivo to affect reproduction. Cultures were prepared from the testis of Sprague-Dawley rats. Cultures containing approximately 3.75 x 10(6) cells/ml were treated with Tl concentrations corresponding to 35, 7, and 1.4-mu-g Tl/g testis, estimated from protein content of cultures. Observations at 24, 48, and 72 h after treatment showed a significant release of germ cells into the culture medium that was both concentration and time dependent. Cultures treated with 34-mu-g Tl/g testis showed a threefold increase in germ-cell detachment compared with controls after only 24 h of exposure. As the treatment time increased to 48 h of exposure, even cultures exposed at the lowest Tl concentration (1.4-mu-g Tl/g testis) showed significant loss of germ cells. After 48 h, cultures exposed to 7-mu-g Tl/g testis exhibited a 2.5-fold increase in germ-cell detachment, and those exposed to 35-mu-g Tl/g testis exhibited a 10-fold increase over controls. Morphological investigations of cell cultures showed evident loss of germ cells with significant reduction in prepachytene and pachytene spermatocytes and changes in the shape of Sertoli cells. These results are in agreement with in vivo studies, in which thallium treatment at comparable exposure levels manifested its earliest toxic testicular effects in Sertoli and germ cells. They also demonstrate the usefulness of this in vitro culture technique to assess toxic testicular damage rapidly.