Sprouty2 Is Associated With Prognosis and Suppresses Cell Proliferation and Invasion in Renal Cell Carcinoma
Sprouty2 Is Associated With Prognosis and Suppresses Cell Proliferation and Invasion in Renal Cell Carcinoma
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DOI：
10.1016/j.urology.2013.02.051
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发表时间：
2013-07-01
期刊：
影响因子：
2.1
通讯作者：
Yin, Changjun
中科院分区：
文献类型：
--
作者：
Li, Pu;Tao, Liangjun;Yin, Changjun
目的探讨Spruty2在肾细胞癌（RCC）中的表达及其临床病理意义，并探讨其在RCC体外增殖和侵袭中的作用。材料与方法采用免疫组化和Western blot方法检测Spruty2在RCC及癌旁正常肾组织中的表达。用小分子干扰核糖核酸稳定转染肾癌组织，使Spry2蛋白表达下降，并通过细胞增殖和transwell实验检测Spry2的作用。结果发现Spry2蛋白在肾癌组织中的表达水平较癌旁正常组织低，表达下调（P
OBJECTIVE To investigate the expression pattern of Sprouty2 (Spry2) and its clinicopathologic significance among patients with renal cell carcinoma (RCC) and to detect its role in proliferation and invasion of RCC in vitro.MATERIALS AND METHODS The expression profile of Spry2 in RCC and matched adjacent noncancerous tissues were detected by immunohistochemistry and Western blot analysis. The expression of Spry2 was depleted by stably transfecting with small, interfering ribonucleic acid and the effects of Spry2 were assessed using the cell proliferation and transwell assay.RESULTS We found Spry2 protein expressed at lower levels and modestly downregulated in cancerous RCC tissues compared with adjacent normal tissue (P