Menstrual- and gender-dependent variations in circulating IL-1 agonists, antagonists, and binding proteins.
Menstrual- and gender-dependent variations in circulating IL-1 agonists, antagonists, and binding proteins.
复制标题
循环 IL-1 激动剂、拮抗剂和结合蛋白的月经和性别依赖性变化。
DOI：
10.1002/jlb.63.1.117
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发表时间：
1998
影响因子：
5.5
通讯作者：
Lynch,EA
中科院分区：
文献类型：
--
作者：
Cannon,JG;Abad,LW;Vannier,E;Lynch,EA
为了验证白细胞介素-1 β（IL-1 β）结合蛋白存在性别差异以及这些结合蛋白影响IL-1 β和IL-1 Ra免疫测定的假设，将125 I标记的IL-1 β加入人血浆样品中，然后进行层析。男性（n= 6）、卵泡期女性（n = 6）和黄体期女性中高分子量峰洗脱的总放射性百分比分别为21.0 ± 0.8、19.1 ± 0.9和18.0 ± 0.8（n= 6;男性vs女性，P= 0.032;卵泡vs黄体，P= 0.035），并与血浆sIL-1 RII浓度相关（r= 0.647，P = 0.007）。血浆IL-1 β免疫反应性与同时的细胞分泌率不一致，部分原因是sIL-1 RII干扰IL-1 β测定。血浆IL-1 Ra水平和细胞分泌率之间的对应关系，观察后，样品的系列稀释。这些结果表明，血浆IL-1 β结合能力在男性和女性之间存在差异，sIL-1 RII是一个主要的影响因素。此外，将血浆IL-1亚型免疫反应性与功能性测量（示踪剂结合）或分离细胞的同时释放相关，可以了解血浆中可能存在的测定干扰。
This study tested the hypotheses that sex-related differences in circulating binding proteins for interleukin-1 β (IL-1 β) exist and that these binding proteins affect immunoassays for IL-1 β and IL-1Ra.125I-labeled IL-1 β was added to human plasma samples, then chromatographed. The percentages of total radioactivity eluting in a high-molecular-weight peak were 21.0 + 0.8 for men (n= 6), 19.1 ± 0.9 for follicular phase women (n= 6), and 18.0 ± 0.8 in luteal phase women (n= 6; men vs. women,P= 0.032; follicular vs. luteal,P= 0.035), and correlated with plasma sIL-1RII concentrations (r= 0.647,P= 0.007). Plasma IL-1 β immunoreactivity did not correspond to concurrent cellular secretion rates due, in part, to interference in the IL-1 β assay by sIL-1RII. Correspondence between plasma IL-1Ra levels and cellular secretion rates was observed only after serial dilutions of the samples. These results indicate that plasma IL-1 β binding capacity differs between men and women and that sIL-1RII is a major contributing factor. Furthermore, relating plasma IL-1 isoform immunoreactivity to functional measures (tracer binding) or concurrent release by isolated cells can lead to insights about assay interferences that may exist in plasma.