Noncontact evaluation of the wetting characteristic of a cell sheet in culture medium
Noncontact evaluation of the wetting characteristic of a cell sheet in culture medium
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DOI：
10.1109/icma.2012.6283384
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发表时间：
2012-08
期刊：
影响因子：
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通讯作者：
N. Tanaka;M. Kaneko;R. Uchida;M. Kondo;M. Yamato;T. Okano
中科院分区：
文献类型：
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作者：
N. Tanaka;M. Kaneko;R. Uchida;M. Kondo;M. Yamato;T. Okano
再生医学在全世界越来越流行。特别是，用于受损角膜上皮的细胞片有望在不久的将来商业化。在细胞培养过程中，细胞片的生存能力和耐受性的评估是非常重要的。迄今为止，质量评价是由专家进行的，没有进行任何数量评价。本研究试图尽可能定量和无创地评估细胞片的润湿特性。有三点需要考虑；(1)电池片易干燥，(2)无污染，(3)薄，厚度小于100 μm。在此基础上，研制了一种具有喷气应用和光学观测单元的非接触式评价系统。本研究发现，在不同的培养条件下，细胞片的润湿特性存在显著差异。
Regenerative medicine is getting popular all over the world. Especially, cell sheets for damaged corneal epithelium are expected to be commercially available in the near future. In cell culture procedure, the assessments of viability and tolerability of cell sheet are important. To date, the qualitative assessments have been performed by specialists without any quantitative evaluation. This study attempted to evaluate the wetting characteristic of cell sheet quantitatively and noninvasively as much as possible. There are three points to be considered; (1) cell sheet is susceptible to dry, (2) is free from a contamination, and (3) is thin with a thickness of less than 100 μm. Under these constraints, a non-contact evaluation system with both an air-jet application and an optical observation units was newly developed. This study found that there is a significant difference in the wetting characteristics of cell sheets in different culture conditions.