Further Studies of Lipid Droplets in the Bombykol‐Producing Pheromone Gland of Bombyx mori
Further Studies of Lipid Droplets in the Bombykol‐Producing Pheromone Gland of Bombyx mori
复制标题
DOI：
10.1196/annals.1327.049
复制
发表时间：
2005-04
影响因子：
5.2
通讯作者：
A. Fónagy;A. Ohnishi;Y. Esumi;Yoshikatsu Suzuki;S. Matsumoto
中科院分区：
文献类型：
--
作者：
A. Fónagy;A. Ohnishi;Y. Esumi;Yoshikatsu Suzuki;S. Matsumoto
摘要：家蚕成虫羽化过程中信息素产生细胞中含有丰富的脂滴，其大小和数量每天都有波动。它们的动态与PBAN刺激的从头产生的蚕豆酚有关。为了阐明相关事件，我们进行了以下工作：(1)提取、纯化和部分鉴定在其表面发现的脂滴相关蛋白，因为它们的功能可能是运输和/或对接负责甘油三酯脂解的可能的脂肪酶；(2)分离、纯化和初步分析脂蛋白携带的脂类和源于幼虫和成虫血淋巴的脂滴转移颗粒，因为它们在脂滴的形成和积累中起作用。
Abstract: Lipid droplets are abundant in the pheromone‐producing cells of B. mori at adult eclosion, followed by daily fluctuations in both their size and number. Their dynamics are related to PBAN‐stimulated de novo bombykol production. To elucidate associated events, we performed the following: (1) extraction, purification, and partial characterization of lipid droplet‐associated proteins found on their surface since their function could possibly be to transport and/or dock putative lipases that are responsible for the lipolysis of triglycerides in them; (2) separation, purification, and initial analysis of lipids carried by lipophorins and lipid transfer particles originating from pupal and adult hemolymph because of their role in the formation and accumulation of lipid droplets.