Divergence of Evolutionary Ways Among Common sym Genes: CASTOR and CCaMK Show Functional Conservation Between Two Symbiosis Systems and Constitute the Root of a Common Signaling Pathway
Divergence of Evolutionary Ways Among Common sym Genes: CASTOR and CCaMK Show Functional Conservation Between Two Symbiosis Systems and Constitute the Root of a Common Signaling Pathway
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DOI：
10.1093/pcp/pcn153
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发表时间：
2008-11-01
影响因子：
4.9
通讯作者：
Imaizumi-Anraku, Haruko
中科院分区：
文献类型：
--
作者：
Banba, Mari;Gutjahr, Caroline;Imaizumi-Anraku, Haruko
近年来，在模式豆科植物莲花和蒺藜苜蓿中发现了许多参与根瘤（RN）共生的豆科植物基因。其中，一组独特的基因被归类为共同共生途径（CSP），因为它们对于另一种相互作用，即丛枝菌根（AM）共生也是至关重要的，该共生在进化上比RN共生更古老，并且广泛分布在植物界中。基于豆科植物 RN 共生是从古代 AM 共生演化而来的概念，一个问题是 CSP 在非结瘤植物（如水稻）和结瘤豆科植物之间是否在功能上保守。我们鉴定了三个水稻CSP基因直系同源物OsCASTOR、OsPOLLUX和OsCCaMK，并证明了OsPOLLUX和OsCCaMK在水稻AM共生中不可或缺的作用。有趣的是，OsCASTOR 或 OsCCaMK 的分子转染可以完全补充相应 Lotus 突变株系中 AM 和 RN 共生的共生缺陷。我们的研究结果不仅为水稻和莲花之间的AM共生提供了保守的遗传基础，而且表明CSP的核心在RN共生的进化过程中得到了很好的保守。经过进化，CASTOR 和 CCaMK 仍然是维持两个共生系统中 CSP 功能的分子基础。
In recent years a number of legume genes involved in root nodule (RN) symbiosis have been identified in the model legumes, Lotus japonicus (Lotus) and Medicago truncatula. Among them, a distinct set of genes has been categorized as a common symbiosis pathway (CSP), because they are also essential for another mutual interaction, the arbuscular mycorrhiza (AM) symbiosis, which is evolutionarily older than the RN symbiosis and is widely distributed in the plant kingdom. Based on the concept that the legume RN symbiosis has evolved from the ancient AM symbiosis, one issue is whether the CSP is functionally conserved between non-nodulating plants, such as rice, and nodulating legumes. We identified three rice CSP gene orthologs, OsCASTOR, OsPOLLUX and OsCCaMK, and demonstrated the indispensable roles of OsPOLLUX and OsCCaMK in rice AM symbiosis. Interestingly, molecular transfection of either OsCASTOR or OsCCaMK could fully complement symbiosis defects in the corresponding Lotus mutant lines for both the AM and RN symbioses. Our results not only provide a conserved genetic basis for the AM symbiosis between rice and Lotus, but also indicate that the core of the CSP has been well conserved during the evolution of RN symbiosis. Through evolution, CASTOR and CCaMK have remained as the molecular basis for the maintenance of CSP functions in the two symbiosis systems.