First isolation and molecular characterization of Ehrlichia canis in Spain
First isolation and molecular characterization of Ehrlichia canis in Spain
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DOI：
10.1016/j.vetpar.2004.08.007
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发表时间：
2004-11-10
影响因子：
2.6
通讯作者：
Tesouro, MA
中科院分区：
文献类型：
--
作者：
Aguirre, E;Sainz, A;Tesouro, MA
本文报道了在西班牙首次用DH82细胞系从一只自然感染的狗身上分离和培养出犬埃利希体。经DNA提取和PCR扩增，获得了新犬E. canis菌株16S rRNA基因的近完整序列（1412 bp）。该菌株核苷酸序列的GenBank登录号为AY394465。该序列与GenBank中其他埃利希体菌株的16S rRNA基因序列一致。本文报道的犬E. canis菌株的16S rRNA基因序列与来自日本、委内瑞拉和以色列等不同地理区域的犬E. canis的16S rRNA基因序列具有很高的相似性。这些数据证实了西班牙存在犬伊氏绦虫。(C) 2004 Elsevier B.V.版权所有
This paper reports the first isolation and culture of Ehrlichia canis in Spain from a naturally infected dog using the DH82 cell line. After DNA extraction and PCR amplification, a nearly complete (1412 bp) sequence of the 16S rRNA gene of the new E. canis strain was obtained. The GenBank accession number for the nucleotide sequence of this strain is AY394465. This sequence was aligned with the 16S rRNA gene sequences of other Ehrlichia strains accessible in GenBank. The 16S rRNA gene sequence of the E. canis strain reported here showed a high percentage of similarity with the 16S rRNA gene sequence of E. canis from different geographic areas including Japan, Venezuela and Israel. These data confirm the presence of E. canis in Spain. (C) 2004 Elsevier B.V. All rights reserved.