Integrative analysis of mRNA and miRNA expression profiles in oral lichen planus: preliminary results
Integrative analysis of mRNA and miRNA expression profiles in oral lichen planus: preliminary results
复制标题
口腔扁平苔藓中 mRNA 和 miRNA 表达谱的综合分析：初步结果
DOI：
10.1016/j.oooo.2017.05.513
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发表时间：
2017-10-01
影响因子：
2.9
通讯作者：
Tang, Guoyao
中科院分区：
文献类型：
--
作者：
Chen, Junjun;Du, Guanhuan;Tang, Guoyao
Objective.口腔扁平苔藓（OLP）是一种病因不明的慢性炎症性疾病，被认为是一种潜在的恶性口腔疾病。本研究的目的是分析OLP患者和健康对照（HC）的候选microRNAs（miRNAs）和基因。从OLP患者（n = 9）和HC患者（n = 4）中采集口腔粘膜活检标本。采用新一代测序技术，结合DESeq和edgeR软件算法筛选差异表达的miRNAs和差异表达基因。共检测到94个差异表达的miRNAs和599个差异表达的基因。通过分析miRNAemessenger RNA网络获得潜在的调节miRNA和基因。其中，与HC相比，5种下调的miRNAs dHsa-miR-135 a-5 p（P =.33）、hsa-miR-128-3 p（P = .03）、hsa-miR-218-5 p（P = .01）、hsa-miR-125 a-5 p（P =.01）和hsa-let-7 e-5 p（P = .04）d是OLP患者中最有希望的生物标志物。通过京都基因与基因组百科全书和基因本体论分析，发现差异表达基因在炎症事件和免疫相关术语中显著富集。mRNA和miRNA表达谱的整合分析为阐明OLP的基因表达机制提供了重要信息，为OLP的病因和发病机制的研究提供了全面的视角。
Objective. Oral lichen planus (OLP), a chronic inflammatory disease of unknown etiology, is considered a potentially malignant oral disorder. The aim of the present study was to analyze candidate microRNAs (miRNAs) and genes from patients with OLP and healthy controls (HCs).Study Design. Biopsy specimens of the oral mucosa were collected from patients with OLP (n = 9) and from HCs (n = 4). Differentially expressed miRNAs and differentially expressed genes were screened by using next-generation sequencing with DESeq and edgeR software algorithms.Results. A total of 94 differentially expressed miRNAs and 599 differentially expressed genes were detected in OLP. Potential regulatory miRNAs and genes were obtained by analyzing miRNAemessenger RNA networks. Of these, 5 downregulated miRNAsdHsa-miR-135 a-5 p (P =.33), hsa-miR-128-3 p (P = .03), hsa-miR-218-5 p (P = .01), hsa-miR-125 a-5 p (P =.01), and hsa-let-7 e-5 p (P = .04) dwere the most promising biomarkers in patients with OLP compared with HCs. The identified differentially expressed genes were significantly enriched in "inflammatory" events and immune-related terms through Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analysis.Conclusions. The integrative analysis of messenger RNA and miRNA profiles provides important information to elucidate gene expression mechanisms and a comprehensive perspective to study the etiology and pathogenesis of OLP.