The Influence of Probiotics on Zebrafish Danio Rerio Innate Immunity and Hepatic Stress
The Influence of Probiotics on Zebrafish Danio Rerio Innate Immunity and Hepatic Stress
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DOI：
10.1089/zeb.2013.0932
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发表时间：
2014-04-01
期刊：
影响因子：
2
通讯作者：
Carnevali, Oliana
中科院分区：
文献类型：
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作者：
Gioacchini, Giorgia;Giorgini, Elisabetta;Carnevali, Oliana
本研究探讨了益生菌对斑马鱼肠道天然免疫和肝脏应激的影响。用益生菌鼠李糖乳杆菌IMC 501（（R））处理成年斑马鱼10天。为了评估分子水平的影响，通过实时聚合酶链反应定量了参与先天免疫系统、应激反应、氧化应激和细胞凋亡的基因的mRNA水平。与对照鱼相比，在益生菌处理的鱼的肠组织中观察到与先天免疫应答相关的生物标志物的增加。此外，在益生菌喂养的鱼的肝脏中观察到压力和抗肿瘤相关基因的丰度减少。最后，对肝脏切片进行成像傅里叶变换红外分析，获得的数据证实益生菌给药降低了氧化应激水平，降低了DNA损伤，并增加了脂质饱和度水平。总体而言，结果表明，益生菌管理可以通过调节先天免疫反应和改善肝脏应激耐受性来增强斑马鱼的福利。
In this study, the effects of probiotic administration on zebrafish Danio rerio intestinal innate immunity and hepatic stress were evaluated. Zebrafish adults were treated for 10 days with the probiotic Lactobacillus rhamnosus IMC 501((R)). To assess the effects at the molecular level, the mRNA levels of genes involved in the innate immune system, stress response, oxidative stress, and apoptosis were quantified by real-time polymerase chain reaction. An increase of biomarkers related to innate immune responses was observed in intestinal tissue from the probiotic-treated fish compared with the control fish. In addition, a decrease in the abundance of stress and apoptotic-related genes was observed in the liver of the probiotic-fed fish. Finally, imaging Fourier transform infrared analysis was conducted on liver sections and the data obtained confirmed that probiotic administration decreased oxidative stress levels, decreased DNA damage, and increased lipid saturation levels. Overall, the results show that probiotic administration may enhance zebrafish welfare by modulating the innate immune response and improving hepatic stress tolerance.