Expression of midkine and its clinical significance in esophageal squamous cell carcinoma
Expression of midkine and its clinical significance in esophageal squamous cell carcinoma
复制标题
DOI：
10.3748/wjg.v12.i13.2006
复制
发表时间：
2006-04-07
影响因子：
4.3
通讯作者：
Zhang, Qing-Yun
中科院分区：
文献类型：
--
作者：
Ren, Ying-Jia;Zhang, Qing-Yun
目的：目的：探讨中期因子（midkine）在食管鳞癌（esophageal squamous cell carcinoma，ESCC）中的表达及其与临床病理特征的关系。结果：RT-PCR和免疫细胞化学均显示中期因子在EC 109细胞中有表达。食管鳞癌组织中有37例（56.1%）呈阳性反应。中期因子表达于肿瘤细胞胞浆。中期因子在肿瘤血管丰富区和肿瘤浸润边缘表达强度较强。中期因子在高分化肿瘤中的表达（76.9%）明显高于中、低分化肿瘤（分别为43.1%和41.2%）（P
Aim: To investigate the expression of midkine in esophageal squamous cell carcinoma (ESCC) and analyze its relationship with clinicopathological features.Methods: RT-PCR and immunocytochemical staining were used to detect the expression of midkine mRNA and protein in EC109 cells, respectively. Then the expression of midkine in 66 cases of ESCC samples were detected by immunohistochemistry using monoclonal antibodies against human midkine.Results: Midkine was expressed in EC109 cell by RTPCR and immunocytochemistry. The immunoreactivity was detected in 56.1% (37/66) of the ESCC samples. The expression of midkine was found in cytoplasm of tumor cells. Notably, the intensity of midkine was stronger at the area abundant in vessels and the invading border of the tumors. Midkine was more intensely expressed in well differentiated tumors (76.9%) than in moderately and poorly differentiated tumors (43.1% and 41.2%, respectively) (P