Combined microscale mechanical topography and chemical patterns on polymer cell culture substrates
Combined microscale mechanical topography and chemical patterns on polymer cell culture substrates
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DOI：
10.1016/j.biomaterials.2005.11.022
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发表时间：
2006-04-01
期刊：
影响因子：
14
通讯作者：
King, WP
中科院分区：
文献类型：
--
作者：
Charest, JL;Eliason, MT;King, WP
本文提出了一种在聚合物电池基片上独立形成机械形貌和表面化学图案的技术。并研究成骨细胞对这些表面图案的反应。图案在两个单独的步骤中形成：热压压印光刻形成机械形貌和微接触印刷产生化学图案。得到的基材具有由4 μ m深和8 μ m宽的压花槽和16 μ m宽的台面以及10 μ m宽的微接触印刷的粘合剂条带组成的表面特征，所述微接触印刷的粘合剂条带的间距为10至100 μ m。当呈现单独的机械形貌或化学图案时，细胞显著地与呈现的图案对齐。当呈现与正交化学图案重叠的机械形貌时，细胞与机械形貌对齐。随着化学图案间距的增加，成骨细胞保持与机械形貌对齐。与传统的基于光刻和湿化学的微加工方法不同，所提出的图案化技术与大量的生物材料兼容，可以形成具有远小于1 μ m的特征的图案，并且高度可扩展到大衬底。(c)2005爱思唯尔有限公司保留所有权利。
This paper presents a technique to independently form mechanical topography and surface chemical patterns oil polymer cell Substrates. and studies the response of osteoblast cells to these surface patterns. The patterns were formed in two separate steps: hot embossing imprint lithography formed the mechanical topography and microcontact printing created the chemical pattern. The resulting substrate had surface features consisting of embossed grooves 4 mu m deep and 8 mu m wide spaced by 16 mu m wide mesas and microcontact printed adhesive lanes 10 mu m wide with spacings that ranged from 10 to 100 mu m. When presented with either mechanical topography or chemical patterns alone, the cells significantly aligned to the pattern presented. When presented with mechanical topography overlaid with an orthogonal chemical pattern, the cells aligned to the mechanical topography. As the chemical pattern spacing was increased, osteoblasts remained aligned to the mechanical topography. Unlike traditional microfabrication approaches based on photolithography and wet chemistry, the patterning technique presented is compatible with a large number of biomaterials, Could form patterns with features much smaller than 1 mu m, and is highly scalable to large substrates. (c) 2005 Elsevier Ltd. All rights reserved.