An accelerated antibody aggregation test based on time sequenced dynamic light scattering
An accelerated antibody aggregation test based on time sequenced dynamic light scattering
复制标题
基于时间序列动态光散射的加速抗体聚集测试
DOI：
10.1016/j.colsurfa.2022.129833
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Velev, Orlin D.
中科院分区：
文献类型：
--
作者：
Conner, Cathryn G.;McAndrew, James;Menegatti, Stefano;Velev, Orlin D.
报道了一种快速测定蛋白质分子在溶液中胶体稳定性的方法，该方法是评价抗体制剂稳定性的有效工具。以人多克隆免疫球蛋白G(Ig G)为模型蛋白，采用动态光散射(DLS)技术测定溶液中颗粒的大小，研究了不同温度和抗体浓度下的聚集速率。为了在提高精度的同时缩短观测周期，开发了一种新的DLS分析方法，该方法包括：(I)高分辨率数据的分布分析，并对溶液中存在的多种颗粒尺寸进行拟合；(Ii)温度上升到中间温度，然后在恒温下进行数小时的应力测试；以及(Iii)用三维曲线图显示选定温度下颗粒尺寸分布的随时间变化的情况。由此得到的3-D曲线图能够稳健地识别不同分散条件下聚集的开始。这种方法可以快速评估温度和浓度等参数对抗体溶液稳定性的影响。
A rapid method for determination of the colloidal stability of protein molecules in solution is reported as an efficient tool for evaluating the stability of antibody formulations. Using human polyclonal immunoglobulin G (IgG) as a model protein and dynamic light scattering (DLS) as a technique to determine the size of particles in solution, the rate of aggregation is investigated at different temperatures and antibody concentrations. To reduce the observation period while increasing precision, a new approach to DLS analysis is developed that comprises: (i) a distribution analysis of high-resolution data, and fitting for multiple particle sizes present in a solution, (ii) a temperature ramp to an intermediate temperature followed by a stress test at constant temperature over several hours, and (iii) 3-D plotting to reveal the time-dependent evolution of the particle size distribution at the selected temperature. The resulting 3-D plots enable robust identification of the onset of aggregation with different dispersion conditions. This method enables rapid evaluation of the effects of parameters such as temperature and concentration on the stability of antibody solutions.
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影响因子：
5.6
作者：
Blake S;Amin S;Qi W;Majumdar M;Lewis EN
通讯作者：
Lewis EN
影响因子：
1.6
作者：
Li, Ye;Lubchenko, Vassiliy;Vekilov, Peter G.
通讯作者：
Vekilov, Peter G.
影响因子：
100.3
作者：
Beck, Alain;Wurch, Thierry;Corvaia, Nathalie
通讯作者：
Corvaia, Nathalie
影响因子：
5.5
作者：
Kanao, M;Matsuda, Y;Sato, T
通讯作者：
Sato, T
溶剂环境对人多克隆IgG在溶液中构象和稳定性的影响
DOI：
10.1016/j.biologicals.2005.06.007
发表时间：
2006
期刊：
Biologicals : journal of the International Association of Biological Standardization
影响因子：
--
作者：
Arpád Szenczi;J. Kardos;G. Medgyesi;P. Závodszky
通讯作者：
P. Závodszky