Clostridium perfringens epsilon toxin is absorbed from different intestinal segments of mice
Clostridium perfringens epsilon toxin is absorbed from different intestinal segments of mice
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DOI：
10.1016/j.toxicon.2008.02.008
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发表时间：
2008-06-01
期刊：
影响因子：
2.8
通讯作者：
Miyakawa, M. E. Fernandez
中科院分区：
文献类型：
--
作者：
Losada-Eaton, D. M.;Uzal, F. A.;Miyakawa, M. E. Fernandez
产气荚膜梭菌肠毒素是一种强效毒素，可引起多种动物的快速致死性肠毒血症。毒素的致病机制包括对内皮细胞和神经元的毒性。虽然肉毒杆菌毒素是从胃肠道吸收的，但毒素吸收的肠道区域和有利于肉毒杆菌毒素吸收的条件尚不清楚。本研究旨在测定经小鼠不同胃肠道吸收的黄曲霉毒素的毒性，并评价肠道环境对黄曲霉毒素吸收的影响。将稀释在几种不同盐水溶液中的一种中的表毒素通过外科手术引入结扎的小鼠胃或肠段。经胃肠道不同部位注射毒素的毒性比较表明，该毒素可经小肠和大肠吸收，但不能经胃吸收。大肠杆菌毒素经结肠注射的致死率高于小肠注射。低pH值、Na+和葡萄糖加入到盐溶液中增加了注射到小肠中的毒素的毒性。这项研究表明，毒素的吸收可以发生在小鼠的任何肠段，肠内容物的理化特性可以影响这种毒素的吸收。(c)2008爱思唯尔有限公司保留所有权利。
Clostridium perfringens epsilon toxin is a potent toxin responsible for a rapidly fatal enterotoxaemia in several animal species. The pathogenesis of epsilon toxin includes toxicity to endothelial cells and neurons. Although epsilon toxin is absorbed from the gastrointestinal tract, the intestinal regions where the toxin is absorbed and the conditions favoring epsilon toxin absorption are unknown. The aim of this paper was to determine the toxicity of epsilon toxin absorbed from different gastrointestinal segments of mice and to evaluate the influence of the intestinal environment in the absorption of this toxin. Epsilon toxin diluted in one of several different saline solutions was surgically introduced into ligated stomach or intestinal segments of mice. Comparison of the toxicity of epsilon toxin injected in different sections of the gastrointestinal tract showed that this toxin can be absorbed from the small and the large intestine but not from the stomach of mice. The lethality of epsilon toxin was higher when this toxin vias injected in the colon than in the small intestine. Low pH, and Na+ and glucose added to the saline solution increased the toxicity of epsilon toxin injected into the small intestine. This study shows that absorption of epsilon toxin can occur in any intestinal segment of mice and that the physicochemical characteristics of the intestinal content can affect the absorption of this toxin. (c) 2008 Elsevier Ltd. All rights reserved.