Properties of pathways that mediate motor learning in the vestibulo-ocular reflex of monkeys.
Properties of pathways that mediate motor learning in the vestibulo-ocular reflex of monkeys.
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介导猴子前庭眼反射运动学习的通路特性。
DOI：
10.1101/sqb.1990.055.01.076
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
Brontë-Stewart,HM
中科院分区：
文献类型：
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作者：
Lisberger,SG;Broussard,DM;Brontë-Stewart,HM
方法实验在清醒的恒河猴身上进行，恒河猴接受过固定和跟踪光点的训练（方法见Wurtz 1969）。训练完成后，用氟烷麻醉每只猴，并使用无菌程序植入几个器械。螺栓固定在头骨上，用于连接一个平台，该平台可以用作眼镜架，并限制猴子的头部（Miles和Eightmy 1980）。在一只眼睛中植入线圈，使我们能够使用磁探测线圈方法来监测水平和垂直的眼睛位置（Judge等，1980）。在第二次手术中，采用经颞叶入路在上级半规管内腔的外淋巴间隙中植入刺激电极（纳尔逊，1982）。使用牙钻通过乳突气房仔细解剖，直到可以看到水平和上级根管。然后使用金刚钻轻轻打磨上级髓腔的骨迷路，直到其足够薄，可以用解剖针钻一个小孔。将电极插入孔中并用Histacryl蓝外科粘合剂和牙科粘固剂固定。我们将电极植入上级神经管，因为它们提供了来自水平神经管的传入神经的极好的电激活，而不会损害水平平面内头部自然转动对该神经管的机械激活（Bront 6-Stewart和Lisberger 1990）。刺激电极被植入三只猴子的上级半规管。在另一只猴子中，刺激电极是
METHODSExperiments were conducted on awake rhesus monkeys that had been trained to fixate and track spots of light (for method, see Wurtz 1969). After training had been completed, each monkey was anesthetized with Halothane, and aseptic procedure was used to implant several devices. Bolts were secured to the skull and used to attach a platform that could be used as a mount for spectacles and to restrain the monkey's head (Miles and Eighmy 1980). A coil of wire was implanted in one eye to allow us to use the magnetic search coil method to monitor horizontal and vertical eye position (Judge et al. 1980).In a second surgery, the postauricular approach was used to implant a stimulating electrode in the perilymphatic space inside the lumen of the superior semicircular canal (Nelson 1982). A dental burr was used to dissect carefully through the mastoid air cells until the horizontal and superior canals could be visualized. A diamond burr was then used to gently sand the bony labyrinth of the superior canal until it was thin enough to make a tiny hole with a dissecting pin. The electrode was inserted into the hole and secured with Histacryl blue surgical adhesive and dental cement. We implanted the electrodes in the superior canal because they provided excellent electrical activation of afferents from the horizontal canal without compromising the mechanical activation of that canal by natural head turns in the horizontal plane (Bront6-Stewart and Lisberger 1990). Stimulating electrodes were implanted in the superior semicircular canals of three monkeys. In one additional monkey, the stimulating electrode was