White collar-1, a circadian blue light photoreceptor, binding to the frequency promoter
White collar-1, a circadian blue light photoreceptor, binding to the frequency promoter
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DOI：
10.1126/science.1073681
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发表时间：
2002-08-02
期刊：
影响因子：
56.9
通讯作者：
Dunlap, JC
中科院分区：
文献类型：
--
作者：
Froehlich, AC;Liu, Y;Dunlap, JC
在粗糙脉孢菌中，蓝光感受器(S)和信号通路(S)尚未被鉴定。我们利用脉孢子虫生物钟的光敏感度，特别是光对时钟分量频率(FRQ)的快速诱导来研究光信号。FRQ的光诱导受转录调控，需要FRQ启动子中的两个顺式作用元件(LRE)。这两种LRE都被含有络合物(WCC)的白领-1(WC-1)/白领-2(WC-2)结合，光会导致与LRE结合的WCC的流动性降低。体外翻译的WC-1和WC-2的使用证实，以黄素腺嘌呤二核苷酸为辅因子的WC-1是蓝光感受器，通过直接与FRQ启动子结合(与WC-2)介导光输入到昼夜节律系统。
In the fungus Neurospora crassa, the blue light photoreceptor(s) and signaling pathway( s) have not been identified. We examined light signaling by exploiting the light sensitivity of the Neurospora biological clock, specifically the rapid induction by light of the clock component frequency (frq). Light induction of frq is transcriptionally controlled and requires two cis-acting elements (LREs) in the frq promoter. Both LREs are bound by a White Collar-1 (WC-1)/White Collar-2 (WC-2) containing complex (WCC), and light causes decreased mobility of the WCC bound to the LREs. The use of in vitro translated WC-1 and WC-2 confirmed that WC-1, with flavin adenine dinucleotide as a cofactor, is the blue light photoreceptor that mediates light input to the circadian system through direct binding( with WC-2) to the frq promoter.