Stromal cells are the main plasminogen activator inhibitor-1-producing cells in human fat - Evidence of differences between visceral and subcutaneous deposits
Stromal cells are the main plasminogen activator inhibitor-1-producing cells in human fat - Evidence of differences between visceral and subcutaneous deposits
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DOI：
10.1161/hq0102.101552
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发表时间：
2002-01-01
影响因子：
8.7
通讯作者：
Alessi, MC
中科院分区：
文献类型：
--
作者：
Bastelica, D;Morange, P;Alessi, MC
在胰岛素抵抗期间观察到的血浆纤溶酶原激活物抑制剂（派）-1升高与主要由内脏脂肪分泌的派-1过量脂肪组织有关。我们的目的是比较派-1在人体内脏和皮下脂肪中的定位。派-1的分泌也在体外人脂肪细胞分化过程中进行了研究。派-1抗原和mRNA定位于组织的基质区，也存在于一些CD 14阳性单核细胞中，与脂肪细胞直接接触。此外，在皮下组织中，派-1 mRNA的含量，通过使用实时聚合酶链反应测定，高于在脂肪细胞组分中的基质组分。派-1 mRNA阳性细胞在内脏区比皮下基质区多5倍（P=0.004）。两种脂肪组织中派-1 mRNA的含量也存在差异。与瘦素相反，在脂肪细胞分化过程中，派-1分泌并不遵循脂肪细胞成熟。原位杂交显示，在培养中没有发现派-1 mRNA的脂质填充细胞。我们的研究结果表明，派-1的生产主要是由于基质细胞，这是更多的内脏比在皮下仓库。这些结果可以解释循环派-1水平与内脏脂肪积累之间的密切关系。
Elevated plasma plasminogen activator inhibitor (PAI)-1 observed during insulin resistance has been connected with an excessive PAI-1 adipose tissue secretion mainly by visceral fat. Our aim was to compare the localization of PAI-1 in human visceral and subcutaneous fats. PAI-1 secretion was also investigated in vitro during human adipocyte differentiation. PAI-1 antigen and mRNA were localized in the stromal area of the tissue and were also present in a few CD14-positive monocytes, in direct contact with adipocytes. In addition, in subcutaneous tissue, PAI-1 mRNA contents, determined by using real-time polymerase chain reaction, were higher in the stromal fraction than in the adipocyte fraction. PAI-1 mRNA-positive cells were 5-fold more frequent in the visceral area than in the subcutaneous stromal area (P=0.004). Such a difference was also observed for PAI-1 mRNA content between both whole adipose tissues. In contrast to leptin, during adipocyte differentiation, PAI-1 secretion did not follow adipocyte maturation. In situ hybridization in culture did not reveal PAI-1 mRNA in lipid-filled cells. Our results demonstrate that PAI-1 production is mainly due to stromal cells, which were more numerous in the visceral than in the subcutaneous depot. These results could explain the strong relationship observed between circulating PAI-1 levels and the accumulation of visceral fat.