Application of a Bacteriophage Lysin To Disrupt Biofilms Formed by the Animal Pathogen Streptococcus suis
Application of a Bacteriophage Lysin To Disrupt Biofilms Formed by the Animal Pathogen Streptococcus suis
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应用噬菌体溶素破坏动物病原体猪链球菌形成的生物膜
DOI：
10.1128/aem.05151-11
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发表时间：
2011-12-01
影响因子：
4.4
通讯作者：
Yan, Yaxian
中科院分区：
文献类型：
--
作者：
Meng, Xiangpeng;Shi, Yibo;Yan, Yaxian
摘要细菌生物膜在许多重要感染的发病机制中起着至关重要的作用，而且很难根除。猪链球菌是猪的重要病原菌，本文对32株猪链球菌的生物被膜能力进行了测定。培养60h后，10株菌完全形成了显著的生物膜，其胞外多糖产量显著高于相应的浮游培养物。扫描电子显微镜显示，猪链霉菌SS2-4菌株形成了致密的生物膜，在这种状态下，与浮游培养物相比，SS2-4菌株对一些抗生素(氨苄西林、阿莫西林、环丙沙星、卡那霉素和利福平)的耐药性增加。一种名为LySMP的噬菌体裂解素被用来单独攻击生物膜，并与抗生素和噬菌体联合使用。结果表明，LySMP对猪链球菌形成的生物膜，特别是SS2-4和SS2-H菌株具有分散作用，与单独使用抗生素或噬菌体相比，其生物膜的降解率可达80%；除破坏生物膜结构外，LySMP对猪链球菌细胞本身也有灭活作用。LySMP的效果不是剂量依赖的，与抗生素联合使用，它可以协同作用，最大限度地分散猪链球菌生物膜，并使释放的细胞失活。这些数据表明，噬菌体裂解酶可能成为治疗猪链球菌感染的有效策略的一部分，并代表了一类新的抗生物膜制剂。
ABSTRACT Bacterial biofilms are crucial to the pathogenesis of many important infections and are difficult to eradicate. Streptococcus suis is an important pathogen of pigs, and here the biofilm-forming ability of 32 strains of this species was determined. Significant biofilms were completely formed by 10 of the strains after 60 h of incubation, with exopolysaccharide production in the biofilm significantly higher than that in the corresponding planktonic cultures. S. suis strain SS2-4 formed a dense biofilm, as revealed by scanning electron microscopy, and in this state exhibited increased resistance to a number of antibiotics (ampicillin, amoxicillin, ciprofloxacin, kanamycin, and rifampin) compared to that of planktonic cultures. A bacteriophage lysin, designated LySMP, was used to attack biofilms alone and in combination with antibiotics and bacteriophage. The results demonstrated that the biofilms formed by S. suis, especially strains SS2-4 and SS2-H, could be dispersed by LySMP and with >80% removal compared to a biofilm reduction by treatment with either antibiotics or bacteriophage alone of less than 20%; in addition to disruption of the biofilm structure, the S. suis cells themselves were inactivated by LySMP. The efficacy of LySMP was not dose dependent, and in combination with antibiotics, it acted synergistically to maximize dispersal of the S. suis biofilm and inactivate the released cells. These data suggest that bacteriophage lysin could form part of an effective strategy to treat S. suis infections and represents a new class of antibiofilm agents.