Dynamic organization of microtubules in guard cells of Vicia faba L. with diurnal cycle
Dynamic organization of microtubules in guard cells of Vicia faba L. with diurnal cycle
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DOI：
10.1093/oxfordjournals.pcp.a029293
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发表时间：
1998-01-01
影响因子：
4.9
通讯作者：
Kondo, N
中科院分区：
文献类型：
--
作者：
Fukuda, M;Hasezawa, S;Kondo, N
气孔运动受保护细胞体积变化的调节，被认为主要受渗透调节系统的控制。在本研究中，我们研究了细胞骨架事件在气孔运动调节中的额外参与，利用免疫荧光显微镜观察了在日光下生长的蚕豆保护细胞中的微管（MTs）。随着气孔孔径的增大，皮层MT在黎明时开始呈放射状排列，在早晨数量增加，随后MT在傍晚至午夜集中在细胞核附近，随着气孔孔径的减小，MT开始被破坏，即使在从自然光条件转为完全黑暗的两天后，MT组织的昼夜变化也能观察到。50 μ M丙酰胺抑制了清晨气孔孔径的增加，破坏了保卫细胞的皮质MTs，而10 μ M紫杉醇则抑制了夜间气孔孔径的减小，稳定了皮层MTs。这些结果表明，保卫细胞的径向组织的皮层MTs可能控制着气孔的昼夜运动。
Stomatal movement is regulated by changes in the volume of guard cells, thought to be mainly controlled by an osmo-regulatory system, In the present study, we examined the additional involvement of cytoskeletal events in the regulation of stomatal movement, Microtubules (MTs) in guard cells of Vicia faba L., grown under sunlight, were observed during the day and night by immunofluorescence microscopy, Cortical MTs began to be organized in a radial array at dawn and increased in numbers in the morning following the increase in the stomatal aperture size, Thereafter, MTs became localized near the nucleus and began to be destroyed from the evening to midnight, following the decrease in stomatal aperture size, These diurnal changes in MT organization were observed even two days after transfer from natural light condition to total darkness, and were accompanied by corresponding changes in stomatal aperture, The increase in stomatal aperture sine in the early morning was inhibited by 50 mu M propyzamide, which destroys cortical MTs in guard cells, whereas the decrease in aperture size in the evening was suppressed by 10 mu M taxol, which stabilizes cortical MTs, These results suggest that radially-organized cortical MTs of guard cells may control diurnal stomatal movement.