Glycyrrhizic Acid Promotes M1 Macrophage Polarization in Murine Bone Marrow-Derived Macrophages Associated with the Activation of JNK and NF-κB.
Glycyrrhizic Acid Promotes M1 Macrophage Polarization in Murine Bone Marrow-Derived Macrophages Associated with the Activation of JNK and NF-κB.
复制标题
DOI：
10.1155/2015/372931
复制
发表时间：
2015
影响因子：
4.6
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Mao Y;Wang B;Xu X;Du W;Li W;Wang Y
甘草属植物的根和根茎已被广泛用作天然甜味剂和草药。本研究旨在探讨甘草甘草酸（GA）对巨噬细胞极化的影响。观察GA对小鼠骨髓源性巨噬细胞（bmdm）的表型和功能活性。结果表明，GA明显增加了CD80、CD86和MHCII分子的细胞表面表达。同时，GA上调CCR7的表达以及经典活化（M1）巨噬细胞的标志物TNF-α、IL-12、IL-6和NO的产生，下调选择性活化（M2）巨噬细胞的标志物MR、Ym1和Arg1的表达。功能实验表明，GA显著增强了BMDMs对fitc -葡聚糖和大肠杆菌K88的摄取，降低了大肠杆菌K88和鼠伤寒沙门氏菌的细胞内存活率。此外，我们证明了JNK和NF-κB激活是ga诱导的NO和m1相关细胞因子产生所必需的，而ERK1/2途径通过诱导IL-10发挥调节作用。综上所述，GA促进了M1巨噬细胞的极化，增强了其吞噬和杀菌能力。这些结果扩大了我们对GA在巨噬细胞极化中的作用的认识。
The roots and rhizomes of Glycyrrhiza species (licorice) have been widely used as natural sweeteners and herbal medicines. The aim of this study is to investigate the effect of glycyrrhizic acid (GA) from licorice on macrophage polarization. Both phenotypic and functional activities of murine bone marrow-derived macrophages (BMDMs) treated by GA were assessed. Our results showed that GA obviously increased the cell surface expression of CD80, CD86, and MHCII molecules. Meanwhile, GA upregulated the expression of CCR7 and the production of TNF-α, IL-12, IL-6, and NO (the markers of classically activated (M1) macrophages), whereas it downregulated the expression of MR, Ym1, and Arg1 (the markers of alternatively activated (M2) macrophage). The functional tests showed that GA dramatically enhanced the uptake of FITC-dextran and E. coli K88 by BMDMs and decreased the intracellular survival of E. coli K88 and S. typhimurium. Moreover, we demonstrated that JNK and NF-κB activation are required for GA-induced NO and M1-related cytokines production, while ERK1/2 pathway exhibits a regulatory effect via induction of IL-10. Together, these findings indicated that GA promoted polarization of M1 macrophages and enhanced its phagocytosis and bactericidal capacity. The results expanded our knowledge about the role of GA in macrophage polarization.