Photosynthetic and phylogenetic primers for detection of anoxygenic phototrophs in natural environments
Photosynthetic and phylogenetic primers for detection of anoxygenic phototrophs in natural environments
复制标题
DOI：
10.1128/aem.67.7.2922-2926.2001
复制
发表时间：
2001-07-01
影响因子：
4.4
通讯作者：
Madigan, MT
中科院分区：
文献类型：
--
作者：
Achenbach, LA;Carey, J;Madigan, MT
设计底漆集以靶向光合细菌的特定16S核糖体DNA（rDNA）序列，包括绿色硫细菌，绿色的非硫细菌和Heliobacteriaceae（Gram阳性门）的成员。由于紫色硫和紫色非氟光植物的系统发育多样性，16S rDNA基因不是系统发育rDNA引物的合适靶标。因此，设计了一个靶向PUFM基因的底漆集，编码了光合作用反应中心的H I亚基，该中心普遍分布在紫色的可营养细菌之间来自氯弗莱克斯物种，它也产生像紫色细菌一样的反应中心。尽管紫色细菌反应中心在结构上类似于绿色植物光系统II，但PUFM引物并未放大蓝细菌DNA，进一步表明了它们对紫色无氧磷脂的特异性，这种质发生和光动力学特异性引物的组合涵盖了所有已知的无氧合光早光果的组组合显示有望是快速评估天然样品中的分子工具这些生物的生态研究。
Primer sets were designed to target specific 16S ribosomal DNA (rDNA) sequences of photosynthetic bacteria, including the green sulfur bacteria, the green nonsulfur bacteria, and the members of the Heliobacteriaceae (a gram-positive phylum). Due to the phylogenetic diversity of purple sulfur and purple nonsulfur phototrophs, the 16S rDNA gene was not an appropriate target for phylogenetic rDNA primers. Thus, a primer set was designed that targets the pufM gene, encoding the h I subunit of the photosynthetic reaction center, which is universally distributed among purple phototrophic bacteria, The pufM primer set amplified DNAs not only from purple sulfur and purple nonsulfur phototrophs but also from Chloroflexus species, which also produce a reaction center like that of the purple bacteria. Although the purple bacterial reaction center structurally resembles green plant photosystem II, the pufM primers did not amplify cyanobacterial DNA, further indicating their specificity for purple anoxyphototrophs, This combination of phyrogenetic- and photosynthesis-specific primers covers all groups of known anoxygenic phototrophs and as such shows promise as a molecular tool for the rapid assessment of natural samples in ecological studies of these organisms.