Properties of the germination inhibitor of Streptomyces viridochromogenes spores.
Properties of the germination inhibitor of Streptomyces viridochromogenes spores.
复制标题
绿色产色链霉菌孢子萌发抑制剂的特性。
DOI：
10.1099/00221287-131-4-833
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Ensign,JC
中科院分区：
文献类型：
--
作者：
Grund,AD;Ensign,JC
绿色产色链霉菌的萌发孢子向周围介质中分泌一种物质，该物质抑制了另一个孢子样品的萌发。发芽抑制剂（GI）是在指数生长停止后在深层培养过程中产生的。用氯仿从生长液中提取后，GI 被纯化 51 倍。它可溶于酒精和水，分子量小于 1000。GI 可以阻断某些革兰氏阳性细菌的生长和呼吸，并且是产生生物体的萌发孢子和菌丝体的膜结合但不溶解的钙依赖性 ATP 酶的抑制剂。几种钠钾激活的 ATP 酶也受到抑制。所有四种活性（呼吸、生长、发芽抑制、ATP 酶）在柱层析和薄层层析过程中共同纯化。发芽期间释放的GI活性和生长期间产生的GI活性相同。讨论了胃肠道抗生素在调节生产生物体孢子休眠中的作用。
Germinating spores ofStreptomyces viridochromogenesexcreted a substance into the surrounding medium which inhibited germination of another sample of the spores. The germination inhibitor (GI) was produced during submerged culture after exponential growth had ceased. The GI was purified 51-fold following extraction from growth liquor with chloroform. It was soluble in alcohol and water and had a molecular weight of less than 1000. The GI blocked growth and respiration of some Gram-positive bacteria and was an inhibitor of the membrane bound, but not solubilized, calcium-dependent ATPase of germinated spores and mycelia of the producing organism. Several sodium–potassium activated ATPases were also inhibited. All four activities (respiration, growth, germination inhibition. ATPase) co-purified during column and thin-layer chromatography. The GI activities released during germination and produced during growth were identical. A role for the GI antibiotic in regulation of dormancy of spores of the producing organism is discussed.