BABESIA-BOVIS - CONTINUOUS CULTIVATION IN A MICRO-AEROPHILOUS STATIONARY PHASE CULTURE
BABESIA-BOVIS - CONTINUOUS CULTIVATION IN A MICRO-AEROPHILOUS STATIONARY PHASE CULTURE
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DOI：
10.1126/science.7355284
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发表时间：
1980-01-01
期刊：
影响因子：
56.9
通讯作者：
RISTIC, M
中科院分区：
文献类型：
--
作者：
LEVY, MG;RISTIC, M
原生动物寄生虫牛巴贝斯虫是牛巴贝斯虫病的病原体，已在牛红细胞的固定层中持续培养。宿主红细胞层内氧张力降低导致受感染培养物颜色变深，并提供了评估寄生虫生长的快速方法。二氧化碳的缺乏导致裂殖子在培养基中积累，而不是侵入新的红细胞。当与培养物分离时，这些红细胞外寄生虫保留其感染性。体外产生的寄生虫在形态上与受感染牛血液中的寄生虫相同，并且对抗巴贝虫药物敏感。
The protozoan parasiteBabesia bovis, a causative agent of bovine babesiosis, has been continuously cultivated in a settled layer of bovine erythrocytes. Lowered oxygen tension within the layer of host erythrocytes results in a darkening of infected cultures and provides a rapid means of evaluating parasite growth. Deprivation of carbon dioxide causes the merozoites to accumulate in the medium rather than invading new erythrocytes. When separated from the culture, these extraerythrocytic parasites retain their infectivity. Parasites produced in vitro are morphologically identical to parasites from the blood of infected cattle and are susceptible to antibabesial drugs.