Role of Cytoskeleton in Controlling the Disorder Strength of Cellular Nanoscale Architecture
Role of Cytoskeleton in Controlling the Disorder Strength of Cellular Nanoscale Architecture
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DOI：
10.1016/j.bpj.2010.05.023
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发表时间：
2010-08-04
影响因子：
3.4
通讯作者：
Backman, Vadim
中科院分区：
文献类型：
--
作者：
Damania, Dhwanil;Subramanian, Hariharan;Backman, Vadim
细胞骨架在细胞中普遍存在，参与细胞运输、信号转导、基因转录、细胞分裂等重要的细胞过程。部分波谱显微镜是一种新型的光学技术，可以从无序强度的角度测量细胞纳米级组织的统计特性。先前已经发现，细胞纳米结构紊乱强度的增加是癌变的最早事件之一。在这项研究中，我们研究了两种形态（因此在显微镜下）相似但基因改变的人类结肠癌细胞系HT29细胞和Csk shrna转染的HT29细胞之间的差异紊乱强度的细胞成分，这两种细胞表现出不同程度的肿瘤侵袭性。为了了解细胞骨架在纳米结构改变中的作用，我们对这些细胞类型的特定细胞骨架成分进行了选择性药物治疗，并研究了细胞骨架组织对无序强度差异的影响。我们报道，通过破坏细胞骨架组织来改变细胞纳米结构导致微观上难以区分的HT29和CSK结构之间无序强度差异的衰减。因此，我们证明细胞骨架在细胞纳米级紊乱的控制中起作用。
Cytoskeleton is ubiquitous throughout the cell and is involved in important cellular processes such as cellular transport, signal transduction, gene transcription, cell-division, etc. Partial wave spectroscopic microscopy is a novel optical technique that measures the statistical properties of cell nanoscale organization in terms of the disorder strength. It has been found previously that the increase in the disorder strength of cell nanoarchitecture is one of the earliest events in carcinogenesis. In this study, we investigate the cellular components responsible for the differential disorder strength between two morphologically (and hence microscopically) similar but genetically altered human colon cancer cell lines, HT29 cells and Csk shRNA-transfected HT29 cells that exhibit different degrees of neoplastic aggressiveness. To understand the role of cytoskeleton in nanoarchitectural alterations, we performed selective drug treatment on the specific cytoskeletal components of these cell types and studied the effects of cytoskeletal organization on disorder strength differences. We report that altering the cell nanoarchitecture by disrupting cytoskeletal organization leads to the attenuation of the disorder strength differences between microscopically indistinguishable HT29 and CSK constructs. We therefore demonstrate that cytoskeleton plays a role in the control of cellular nanoscale disorder.