The role of fibronectin in adhesion of metastatic melanoma cells to endothelial cells and their basal lamina.
The role of fibronectin in adhesion of metastatic melanoma cells to endothelial cells and their basal lamina.
复制标题
纤连蛋白在转移性黑色素瘤细胞与内皮细胞及其基底层粘附中的作用。
DOI：
10.1016/0014-4827(81)90191-9
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发表时间：
1981
影响因子：
3.7
通讯作者：
Ruoslahti,E
中科院分区：
文献类型：
--
作者：
Nicolson,GL;Irimura,T;Gonzalez,R;Ruoslahti,E
血管内皮细胞合成由胶原、蛋白聚糖和糖蛋白如纤连蛋白（FN）组成的细胞外基质或基底层。使用亲和纯化的抗FN，我们已经研究了FN的作用，在粘附的转移性B16黑色素瘤细胞的内皮细胞单层缺乏FN的顶端细胞表面和其基底层，其中包含FN。B16黑色素瘤细胞，其中不包含显着量的FN，附着在更高的利率内皮基膜和聚乙烯固定的FN相比，完整的内皮细胞单层。抗FN不能抑制低（B16-F1）或高（B16-F10）转移潜能的黑色素瘤亚系与完整内皮细胞单层的附着，轻微抑制B16细胞与基底层的附着，并完全消除B16细胞与聚乙烯固定的FN的附着。抑制B16细胞表面糖蛋白糖基化并阻断实验转移的抗生素衣霉素[18]抑制B16与内皮细胞、基底层和固定化FN的附着。结果表明，FN介导，只有部分，通过糖蛋白受体的B16黑色素瘤细胞的粘附到基底层。
Vascular endothelial cells synthesize an extracellular matrix or basal lamina composed of collagens, proteoglycans and glycoproteins such as fibronectin (FN). Using affinity-purified anti-FN, we have examined the role of FN in adherence of metastatic B16 melanoma cells to endothelial cell monolayers which lack FN on apical cell surfaces and to their basal lamina which contains FN. B16 melanoma cells, which do not contain significant amounts of FN, attached at much higher rates to endothelial basal lamina and polyvinyl-immobilized FN compared with intact endothelial cell monolayers. Anti-FN failed to inhibit attachment of melanoma sublines of low (B16-F1) or high (B16-F10) metastatic potential to intact endothelial cell monolayers, inhibited slightly B16 cell attachment to basal lamina and completely abolished attachment of B16 cells to polyvinyl-immobilized FN. The antibiotic tunicamycin which inhibits glycosylation of B16 cell surface glycoproteins and blocks experimental metastasis [18] inhibited B16 attachment to endothelial cells, basal lamina and immobilized FN. The results suggest that FN mediates, only in part, the adhesion of B16 melanoma cells to basal lamina through glycoprotein receptors on B16 cells.