Potential identity of multi-potential cancer stem-like subpopulation after radiation of cultured brain glioma
Potential identity of multi-potential cancer stem-like subpopulation after radiation of cultured brain glioma
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DOI：
10.1186/1471-2202-9-15
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发表时间：
2008-01-30
期刊：
影响因子：
2.4
通讯作者：
Kang, Soo K.
中科院分区：
文献类型：
--
作者：
Kang, Mi K.;Hur, Beong I.;Kang, Soo K.
背景：多形性胶质母细胞瘤（GBM）是最常见的脑癌。尽管之前已经确定GBM中存在癌症干细胞，但几乎没有证据可以解释GBM中癌症干细胞和放射抗性细胞之间的差异。为了加深对细胞放射抗性是否是与癌症干细胞相关的特征的理解，我们开发了一种源自GBM的分离细胞亚群系统，并证明了其中的放射治疗抗性。结果：GBM的放射抗性癌细胞亚群大量表达CD133、CD117、CD71和CD45表面标志物，这些抗放射癌细胞亚群具有广泛增殖、自我更新和增殖的能力。多能性。这些抗辐射癌症亚群已被证明在移植到 SCID 小鼠大脑中时会引发肿瘤发生。此外，这些肿瘤表现出高度奇特的巢状形状，含有血管和癌组织结构，表达血管特异性标记物，冯维勒布兰德因子。因此，GBM 中的抗辐射细胞亚群已被证明与循环血液中的造血干细胞 (HSC) 非常相似。这种相似性可能导致肿瘤生长增加和 GBM 复发。结论：本研究的结果为 GBM 中癌症干细胞的放射抗性亚群提供了进一步的证据。此外，我们的结果将有助于神经胶质瘤中癌症干细胞群的识别和表征，并将有助于改善 GBM 的治疗结果。
Background: Glioblastoma multiforme (GBM) is the most frequently encountered brain cancer. Although the existence of cancer stem cells in GBM has been previously established, there is little evidence to explain the difference between cancer stem cells and radio-resistant cells in GBM. In an effort to increase our understanding of whether cellular radio-resistance is a characteristic associated with cancer stem cells, we developed a dissociated cell system of subpopulations derived from GBM, and demonstrated radiotherapy resistance therein.Results: The radio-resistant cancer cell subpopulations of GBM abundantly express CD133, CD117, CD71, and CD45 surface markers, and these radio-resistant cancer cell subpopulations have the capacity for extensive proliferation, self-renewal, and pluripotency. These radio-resistant cancer subpopulations have been shown to initiate tumorigenesis when transplanted into SCID mouse brains. Moreover, these tumors evidenced highly peculiar nest-like shapes harboring both vascular and cancerous tissue structures, which expressed the blood vessel specific marker, the von Willebrand factor. Accordingly, subpopulations of radio-resistant cells in GBM have been shown to be very similar to hematopoietic stem cells (HSCs) in the circulating blood. This similarity may contribute to increased tumor growth and GBM recurrence.Conclusion: The results of the present study provide further evidence for radio resistant subpopulations of cancer stem cells in GBM. Also, our results will assist in the identification and characterization of cancer stem cell populations in glioma, and will help to improve the therapeutic outcomes of GBM.