Protocatechuic acid reduces H2O2-induced migration and oxidative stress of fibroblast-like synoviocytes in rheumatoid arthritis by activating Nrf2-Keap1 signaling pathway
Protocatechuic acid reduces H2O2-induced migration and oxidative stress of fibroblast-like synoviocytes in rheumatoid arthritis by activating Nrf2-Keap1 signaling pathway
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DOI：
10.4103/cjop.cjop-d-22-00087
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发表时间：
2023-01
影响因子：
1.8
通讯作者：
Yan Liu;Yucheng Zhang;Kecheng Zhang;Yue Wang
中科院分区：
文献类型：
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作者：
Yan Liu;Yucheng Zhang;Kecheng Zhang;Yue Wang
蜂巢（Nidus vespae）是传统中药，可治疗类风湿性关节炎（RA），而原儿茶酸（PCA）是蜂巢的生物活性成分。本研究旨在探讨 PCA 是否可以减少 H2O2 诱导的 RA 成纤维细胞样滑膜细胞 (RA-FLS) 的迁移和氧化应激。 H2O2 诱导的 RA-FLS 用于模拟 RA 的体外模型。通过细胞计数试剂盒8（CCK-8）、末端脱氧核苷酸转移酶dUTP缺口末端标记法、伤口愈合、transwell实验、DCFDA染色以及丙二醛和超氧化物歧化酶酶联免疫吸附测定试剂盒检测RA-FLS的活力、凋亡、迁移、侵袭和氧化应激。通过western blotting分析迁移和侵袭相关蛋白以及Nrf2/Keap1信号通路相关蛋白的表达。结果，PCA 通过激活 Nrf2/Keap1 信号通路抑制 H2O2 诱导的 RA-FLS 的活力、迁移、侵袭和氧化并促进其凋亡。 ML-385 是一种 Nrf2 抑制剂，可以增强 H2O2 诱导的 RA-FLS 的活力、迁移、侵袭和氧化，并抑制细胞凋亡。总之，PCA 通过激活 Nrf2-Keap1 信号通路减少 H2O2 诱导的 RA-FLS 迁移和氧化应激。
Honeycomb (Nidus vespae) is traditional Chinese medicine and can treat rheumatoid arthritis (RA), and protocatechuic acid (PCA) is a bioactive component of honeycomb. This study aimed to investigate whether PCA could reduce the H2O2-induced migration and oxidative stress of RA fibroblast-like synoviocytes (RA-FLSs). H2O2-induced RA-FLSs were used to simulate the in vitro model of RA. The viability, apoptosis, migration, invasion, and oxidative stress of RA-FLSs were detected by Cell Counting Kit-8 (CCK-8), terminal deoxynucleotidyl transferase dUTP nick-end labeling assay, wound healing, transwell assays, DCFDA staining, and malonaldehyde and superoxide dismutase enzyme-linked immunosorbent assay kits. The expression of migration and invasion-related proteins and Nrf2/Keap1 signaling pathway-related proteins was analyzed by western blotting. As a result, PCA suppressed the viability, migration, invasion, and oxidative and promoted apoptosis of H2O2-induced RA-FLSs by activating the Nrf2/Keap1 signaling pathway. ML-385, an Nrf2 inhibitor, could enhance the viability, migration, invasion, and oxidative and inhibited apoptosis of H2O2-induced RA-FLSs. In conclusion, PCA reduced H2O2-induced migration and oxidative stress of RA-FLSs by activating the Nrf2-Keap1 signaling pathway.