Quantification and facilitated comparison of von Willebrand factor multimer patterns by densitometry.
Quantification and facilitated comparison of von Willebrand factor multimer patterns by densitometry.
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通过密度测定法对冯维勒布兰德因子多聚体模式进行量化和促进比较。
DOI：
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发表时间：
2001
影响因子：
3.5
通讯作者：
Monika Barthels
中科院分区：
文献类型：
--
作者：
Jan D. Studt;Ulrich Budde;Reinhard Schneppenheim;Roswit Eisert;M. Prondzinski;Arnold Ganser;Monika Barthels
The analysis of von Willebrand factor (vWF) multimers is an important laboratory tool for distinguishing among the numerous subtypes of von Willebrand disease (vWD). Comparability and reproducibility of this method are insufficient; standardization and external references are pending. Interlaboratory comparison of results therefore may be difficult. We applied densitometry to obtain a reproducible quantification of vWF multimer patterns in healthy donors, patients with vWD variants, and factor VIII/vWF concentrates to improve the reproducibility and comparability of vWF multimer analysis. Multimers were separated and visualized luminographically on x-ray films. Films were scanned and evaluated by densitometry. The variation inherent in vWF multimer analysis and the range of the normal could be quantified. In vWD variants and factor VIII/vWF concentrates, densitometry could quantify and visualize alterations of vWF multimer patterns and facilitate their comparison. Densitometry permits a precise quantitative comparison of sample patterns to a reference plasma. It could be a valuable tool offering reproducible quantification and additional visualization of normal and pathologic vWF multimer patterns, facilitating their comparison and contributing to a standardization of vWF multimer analysis.
冯维勒布兰德病“Vicenza”，具有大于正常（超正常）冯维勒布兰德因子多聚体。
影响因子：
20.3
作者：
Mannucci,PM;Lombardi,R;Castaman,G;Dent,JA;Lattuada,A;Rodeghiero,F;Zimmerman,TS
通讯作者：
Zimmerman,TS
血小板计数升高是血浆中冯维勒布兰德因子多聚体分布异常的原因。
影响因子：
20.3
作者：
Budde,U;Scharf,RE;Franke,P;Hartmann-Budde,K;Dent,J;Ruggeri,ZM
通讯作者：
Ruggeri,ZM