Estimation of methanogenesis by quantification of coenzyme F430 in marine sediments
Estimation of methanogenesis by quantification of coenzyme F430 in marine sediments
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DOI：
10.2343/geochemj.2.0410
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发表时间：
2016-01-01
影响因子：
0.8
通讯作者：
Ohkouchi, Naohiko
中科院分区：
文献类型：
--
作者：
Kaneko, Masanori;Takano, Yoshinori;Ohkouchi, Naohiko
辅酶F430是甲基辅酶M还原酶(MCR)的活性部位。MCR-F430络合物催化甲烷生成的最后一步：将甲基辅酶M还原为甲烷。由于F430是一种常见的产甲烷菌的酶，它可以作为一种功能特异的生物标记物来估计环境样品中产甲烷菌的生物量和活性。最近开发的一种高灵敏度的辅酶F430分析方法和进一步的纯化步骤显示，在岛下半岛海底以下69米和88米处的F430浓度(529和31.3fmolg-WEW(-1))，以及南开海槽的60和275 mBsf(31.4和26fmolg-WEW(-1))。根据已报道的产甲烷菌中F430的含量和活性以及原核细胞重量估计的产甲烷生物量和活性在3.9+/-10(6)到2.7+/-10(6)个细胞g-WET(-1)和1.7-11mmold(-1)g-WEW(-1)之间。
Coenzyme F430 is the hydrocorphinoid nickel complex which acts as active site in methyl-coenzyme M reductase (MCR). The MCR-F430 complex catalyzes the last step of methanogenesis: reduction of methyl-coenzyme M to methane. Since F430 is a common enzyme for mathanogens, it can be a function-specific biomarker to estimate biomass and activities of methanogens in environmental samples. A recently developed high sensitive method for coenzyme F430 analysis and a further purification step revealed F430 concentrations at 69 and 88 meters below seafloor (mbsf) off Shimokita Peninsula (529 and 31.3 fmol g-wet(-1)) and at 60 and 275 mbsf at Nankai Trough (31.4 and 26 fmol g-wet(-1)). Estimated methanogenic biomass and activities based on reported F430 content and activities in methanogens and prokaryotic cell weight, were ranging from 3.9 +/- 10(6) to 2.7 +/- 10(6) cells g-wet(-1) and from 1.7 to 11 mu mol d(-1) g-wet(-1).