Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages
Genome-wide identification of the SPL gene family in Tartary Buckwheat (Fagopyrum tataricum) and expression analysis during fruit development stages
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DOI：
10.1186/s12870-019-1916-6
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发表时间：
2019-07-08
影响因子：
5.3
通讯作者：
Chen, Hui
中科院分区：
文献类型：
--
作者：
Liu, Moyang;Sun, Wenjun;Chen, Hui
spl （SQUAMOSA启动子结合蛋白样）是一类植物特异性转录因子，在茎和花序分支、胚胎发育、信号转导、叶片起始、相变以及花和果实发育等许多生长发育过程中发挥重要作用。SPL基因家族已在许多物种中被鉴定和鉴定，但在重要的食药作物苦荞中尚未得到很好的研究。结果本研究共鉴定出24个鞑靼金荞麦（Fagopyrum tararicum, FtSPL）基因，并根据FtSPL基因的染色体分布进行了重命名。根据SBP结构域的氨基酸序列和基因结构，通过系统发育树分析将SPL基因分为I ~ VII组。在苦荞SPL基因中共检测到10个基序。采用实时荧光定量聚合酶链反应（qRT-PCR）测定了23个SPL基因在不同组织和果实不同发育阶段（青果期、变色期和初熟期）的表达谱。结论苦荞基因组包含24个SPL基因，大部分基因在不同组织中表达。qRT-PCR结果显示，FtSPLs在苦荞的生长发育中发挥重要作用，初步鉴定出可能调控花和果实发育的基因。该工作为全面了解苦荞SBP-box基因家族提供了基础，为进一步研究FtSPL基因的功能特征和苦荞作物改良奠定了重要基础。
BackgroundSPL (SQUAMOSA promoter binding protein-like) is a class of plant-specific transcription factors that play important roles in many growth and developmental processes, including shoot and inflorescence branching, embryonic development, signal transduction, leaf initiation, phase transition, and flower and fruit development. The SPL gene family has been identified and characterized in many species but has not been well studied in tartary buckwheat, which is an important edible and medicinal crop.ResultsIn this study, 24 Fagopyrum tataricum SPL (FtSPL) genes were identified and renamed according to the chromosomal distribution of the FtSPL genes. According to the amino acid sequence of the SBP domain and gene structure, the SPL genes were divided into eight groups (group I to group VII) by phylogenetic tree analysis. A total of 10 motifs were detected in the tartary buckwheat SPL genes. The expression patterns of 23 SPL genes in different tissues and fruits at different developmental stages (green fruit stage, discoloration stage and initial maturity stage) were determined by quantitative real-time polymerase chain reaction (qRT-PCR).ConclusionsThe tartary buckwheat genome contained 24 SPL genes, and most of the genes were expressed in different tissues. qRT-PCR showed that FtSPLs played important roles in the growth and development of tartary buckwheat, and genes that might regulate flower and fruit development were preliminarily identified. This work provides a comprehensive understanding of the SBP-box gene family in tartary buckwheat and lays a significant foundation for further studies on the functional characteristics of FtSPL genes and improvement of tartary buckwheat crops.