Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance.
Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance.
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DOI：
10.1016/j.biomaterials.2018.05.045
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发表时间：
2018-05
期刊：
影响因子：
14
通讯作者：
K. Schneider;M. Enayati;C. Grasl;I. Walter;L. Budinsky;Gabriel Zebic;C. Kaun;Anja Wagner;Klaus Kratochwill;H. Redl;A. Teuschl;B. Podesser;H. Bergmeister
中科院分区：
文献类型：
--
作者：
K. Schneider;M. Enayati;C. Grasl;I. Walter;L. Budinsky;Gabriel Zebic;C. Kaun;Anja Wagner;Klaus Kratochwill;H. Redl;A. Teuschl;B. Podesser;H. Bergmeister
Small diameter vascular grafts from human placenta, decellularized with either Triton X-100 (Triton) or SDS and crosslinked with heparin were constructed and characterized. Graft biochemical properties, residual DNA, and protein composition were evaluated to compare the effect of the two detergents on graft matrix composition and structural alterations. Biocompatibility was testedin vitroby culturing the grafts with primary human macrophages andin vivoby subcutaneous implantation of graft conduits (n = 7 per group) into the flanks of nude rats. Subsequently, graft performance was evaluated using an aortic implantation model in Sprague Dawley rats (one month, n = 14).In situgraft imaging was performed using MRI angiography. Retrieved specimens were analyzed by electromyography, scanning electron microscopy, histology and immunohistochemistry to evaluate cell migration and the degree of functional tissue remodeling.Both decellularization methods resulted in grafts of excellent biocompatibilityin vitroandin vivo,with low immunogenic potential. Proteomic data revealed removal of cytoplasmic proteins with relative enrichment of ECM proteins in decelluarized specimens of both groups. Noteworthy, LC-Mass Spectrometry analysis revealed that 16 proteins were exclusively preserved in Triton decellularized specimens in comparison to SDS-treated specimens. Aortic grafts showed high patency rates, no signs of thrombus formation, aneurysms or rupture. Conduits of both groups revealed tissue-specific cell migration indicative of functional remodeling.This study strongly suggests that decellularized allogenic grafts from the human placenta have the potential to be used as vascular replacement materials. Both detergents produced grafts with low residual immunogenicity and appropriate mechanical properties. Observed differences in graft characteristics due to preservation method had no impact on successfulin vivoperformance in the rodent model.
