A nano-graphite-DNA hybrid sensor for magnified fluorescent detection of mercury(II) ions in aqueous solution.
A nano-graphite-DNA hybrid sensor for magnified fluorescent detection of mercury(II) ions in aqueous solution.
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DOI：
10.1039/c3an01482g
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发表时间：
2014-03
期刊：
影响因子：
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通讯作者：
Yin Wei;Bianmiao Li;Xu Wang-;Y. Duan
中科院分区：
文献类型：
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作者：
Yin Wei;Bianmiao Li;Xu Wang-;Y. Duan
在这篇通讯中，我们首次提出了一种纳米石墨- dna混合传感器，用于荧光检测水溶液中的汞（II）离子。此外，还演示了基于纳米石墨的dna酶I检测Hg（2+）的扩增策略。该方法对Hg（2+）的检出限（LOD）为0.5 nM，比传统的非扩增均相分析方法低约20倍。我们进一步展示了它在实际样品中检测Hg（2+）的实际应用。
In this communication, we present a nano-graphite-DNA hybrid sensor for fluorescent detection of mercury(II) ions in aqueous solution for the first time. Furthermore, an amplification strategy based on nano-graphite for Hg(2+) detection by using DNase I was demonstrated. The proposed amplified assay was simple and cost-effective with a limit of detection (LOD) for Hg(2+) of 0.5 nM, which was about 20-fold lower than that of traditional unamplified homogeneous assays. We further demonstrated its practical application to detect Hg(2+) in a real sample.