Green-colored Plastids in the Dinoflagellate Genus Lepidodinium are of Core Chlorophyte Origin
Green-colored Plastids in the Dinoflagellate Genus Lepidodinium are of Core Chlorophyte Origin
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DOI：
10.1016/j.protis.2010.07.001
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发表时间：
2011-04-01
期刊：
影响因子：
2.5
通讯作者：
Inagaki, Yuji
中科院分区：
文献类型：
--
作者：
Matsumoto, Takuya;Shinozaki, Fumihiko;Inagaki, Yuji
大多数光合甲藻具有含叶绿素a+c的质体，但属于Lepidodinium属的物种是唯一的轴承非典型质体含叶绿素a+B。根据关于色素组成数据的开创性工作，已经提出Lepidodinium质体来源于prasinophyte物种，尽管该假设没有得到最近基于由八种质体蛋白组成的比对的系统发育分析的支持（Takishita等人，2008，Gene 410：26-26）。然而，这种“8-蛋白质”分析不足以澄清Lepidodinium质体的起源，原因有两个：首先，比对缺乏足够的进化信息来解析Lepidodinium质体的精确起源。第二，所考虑的类群不能很好地代表绿藻门的多样性。特别是，prasinophytes在对齐中采样不足。在本研究中，我们对L.叶绿素，一个地生植物物种，一个石腐植物物种和六个Prasinophyte物种。本研究中新确定的85个序列和质体基因组测序的最新进展使我们能够制备由来自绿色藻类分类群的11个质体蛋白组成的比对，这些蛋白适当地涵盖了绿藻门的多样性。对11个蛋白质数据集的所有分析都稳健地将L. chlorophorum与“核心绿藻”的成员。“因此，我们在这里提出Lepidodinium质体是核心绿藻起源的。(C)2010年Elsevier GmbH。All rights reserved.
Most photosynthetic dinoflagellates possess plastids containing chlorophylls a+c, but species belonging to the genus Lepidodinium are unique in bearing non-canonical plastids containing chlorophylls a+b. According to the pioneering works on pigment composition data, it has been proposed that Lepidodinium plastids were derived from a prasinophyte species, though this hypothesis was not supported by a recent phylogenetic analysis based on an alignment comprised of eight plastid proteins (Takishita et al. 2008, Gene 410: 26-26). This "8-protein" analysis however was insufficient to clarify the origin of Lepidodinium plastids for two major reasons: First, the alignment lacked sufficient evolutionary information to resolve the precise origin of Lepidodinium plastids. Second, the taxa considered did not well represent the diversity of Chlorophyta. Particularly, prasinophytes were poorly sampled in the alignment. In this study, we sequenced plastid-encoded genes from L. chlorophorum, one pedinophyte species, one ulvophyte species, and six prasinophyte species. The 85 sequences newly determined in this study and recent progress in plastid genome sequencing enabled us to prepare an alignment comprised of 11 plastid proteins from green algal taxa that appropriately cover the diversity of Chlorophyta. All the analyses of the 11-protein data set robustly grouped L. chlorophorum with members of the "core chlorophytes." Thus, we here propose that Lepidodinium plastids are of core chlorophyte origin. (C) 2010 Elsevier GmbH. All rights reserved.