STREAMING POTENTIALS IN CHEMICALLY MODIFIED BONE
STREAMING POTENTIALS IN CHEMICALLY MODIFIED BONE
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DOI：
10.1002/jor.1100060306
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发表时间：
1988-05-01
影响因子：
2.8
通讯作者：
WILLIAMS, WS
中科院分区：
文献类型：
--
作者：
OTTER, M;GOHEEN, S;WILLIAMS, WS
直接流实验，没有显着的机械变形已经进行，以确定主要负责生产的流动电位的牛胫骨的成分或成分。按照Frank和Grozinsky（5）的方法，通过化学方法选择性去除组织的主要成分胶原或羟基磷灰石，并重新测量流动电位。通过在甲酸/柠檬酸钠中浸泡来制备脱矿质样品，并且通过煮沸和次氯酸钠处理来制备无机样品。脱矿物质的样品证明了。电位接近于全骨样品的电位，而无机样品具有小得多的ζ。潜力因此，胶原而不是羟基磷灰石矿物质被认为是支配流动电位的整个骨的成分，这与不含矿物质相的胶原组织的观察结果一致，即，肌腱和软骨也表现出流动电位。没有迹象的变化，观察到在高高NaCl浓度的溶液中含有钙或在低pH值的全骨。虽然标志的信号为无机骨可以通过添加Ca 2+或PO 43+的测试溶液中逆转，信号的迹象，无论是在全骨，也没有脱钙骨可以如此影响。仅在含有碱性有机分子的溶液中观察到全骨的体征变化，硫酸鱼精蛋白
Direct streaming experiments with no significant mechanical deformation have been performed to determine the constituent or constituents of bovine tibia mainly responsible for the production of streaming potentials. Following the approach of Frank and Grozinsky (5), selective removal of either main constituent.sbd.collagen or hydroxyapatite.sbd.of the tissue was performed by chemical means, and the streaming potential was remeasured. Demineralized samples were prepared by soaking in formic acid/sodium citrate, and anorganic samples were prepared by boiling and also by sodium hypochlorite treatment. Demineralized samples demonstrated .zeta. potentials close to those of whole bone samples, whereas anorganic samples had much smaller .zeta. potentials. Collagen rather than hydroxypatite mineral is therefore implicated as the constituent of whole bone dominating the streaming potential, in agreement with the observation that collagenous tissues containing no mineral phase, i.e., tendon and cartilage, also exhibit streaming potentials. No sign change was observed in whole bone at high high NaCl concentration in solutions containing calcium or at low pH. Although the sign of the signal for anorganic bone could be reversed by the addition of Ca2+ or PO43+ to the test solutions, the sign of the signal in neither whole bone nor demineralized bone could be so affected. Sign changes in whole bone were observed only with solutions containing basic organic molecules, e.g., protamine sulphates.