Imbalance of M1/M2 macrophages is linked to severity level of knee osteoarthritis
Imbalance of M1/M2 macrophages is linked to severity level of knee osteoarthritis
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DOI：
10.3892/etm.2018.6852
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发表时间：
2018-12-01
影响因子：
2.7
通讯作者：
Yang, Hao
中科院分区：
文献类型：
--
作者：
Liu, Baolong;Zhang, Maoquan;Yang, Hao
巨噬细胞，无论是 M1 还是 M2 亚型，已被发现与骨关节炎 (OA) 的发病机制有关。然而，尚无关于膝关节 OA 中 M1 和 M2 巨噬细胞状态的研究报道。为了调查膝骨关节炎中 M1 和 M2 巨噬细胞的状态，收集了 80 名膝骨关节炎患者和 80 名健康对照者的滑液和外周血。采用逆转录定量PCR定量检测滑液中M1巨噬细胞标记物CD11c和M2巨噬细胞标记物CD206的表达。作为确认，使用流式细胞术使用单核细胞上的 CD86 (M1) 和 CD163 (M2) 标记来计数全血中的单核细胞数量。膝骨关节炎中M1与M2巨噬细胞的比例明显高于对照组，且该比例与膝骨关节炎的Kellgren-Lawrence分级水平显着正相关，即比例越高，膝骨关节炎似乎越严重。因此，我们的研究提供了巨噬细胞参与膝关节骨关节炎发病机制的直接证据。
Macrophages, whether M1 or M2 subtype, have been found to be implicated in the pathogenesis of osteoarthritis (OA). However, no study regarding the status of M1 and M2 macrophages has been reported in knee OA. To investigate the status of M1 and M2 macrophages in knee OA, synovial fluid as well as peripheral blood were collected from 80 patients with knee OA and 80 healthy controls. Reverse transcription-quantitative PCR was used to quantitatively detect the expression of CD11c as a marker for M1 macrophages and CD206 as a marker for M2 macrophages from synovial fluids. As confirmation, flow cytometry was employed to count the number of monocytes from whole blood using the CD86 (M1) and CD163 (M2) markers on monocytes. The ratio of M1 to M2 macrophages was shown to be markedly higher in knee OA than that of control and that the ratio was significantly positively correlated with level of Kellgren-Lawrence grade in knee OA, that is, the higher the ratio the more severe the knee OA seems to be. Thus, our study presented direct evidence for the involvement of macrophages in the pathogenesis of knee OA.