SYNAPTOTAGMIN - A CALCIUM SENSOR ON THE SYNAPTIC VESICLE SURFACE
SYNAPTOTAGMIN - A CALCIUM SENSOR ON THE SYNAPTIC VESICLE SURFACE
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DOI：
10.1126/science.1589771
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发表时间：
1992-05-15
期刊：
影响因子：
56.9
通讯作者：
JAHN, R
中科院分区：
文献类型：
--
作者：
BROSE, N;PETRENKO, AG;JAHN, R
神经元通过突触小泡的钙依赖性胞吐作用释放神经递质。然而，将钙信号转导为膜融合的分子步骤仍然是一个谜。据报道，突触结合蛋白是一种高度保守的突触小泡蛋白，在生理浓度下与带负电的磷脂复合物中的钙结合。这种结合对钙具有特异性，并涉及突触结合蛋白的细胞质结构域。钙结合依赖于突触结合蛋白的完整寡聚结构（它被单个位点的蛋白水解裂解所消除）。这些结果表明突触结合蛋白在胞吐作用中充当协同钙受体。
Neurons release neurotransmitters by calcium-dependent exocytosis of synaptic vesicles. However, the molecular steps transducing the calcium signal into membrane fusion are still an enigma. It is reported here that synaptotagmin, a highly conserved synaptic vesicle protein, binds calcium at physiological concentrations in a complex with negatively charged phospholipids. This binding is specific for calcium and involves the cytoplasmic domain of synaptotagmin. Calcium binding is dependent on the intact oligomeric structure of synaptotagmin (it is abolished by proteolytic cleavage at a single site). These results suggest that synaptotagmin acts as a cooperative calcium receptor in exocytosis.