PROTONIC CONDUCTANCE ACROSS PHOSPHOLIPID BILAYER MEMBRANES INDUCED BY UNCOUPLING AGENTS FOR OXIDATIVE PHOSPHORYLATION
PROTONIC CONDUCTANCE ACROSS PHOSPHOLIPID BILAYER MEMBRANES INDUCED BY UNCOUPLING AGENTS FOR OXIDATIVE PHOSPHORYLATION
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DOI：
10.1073/pnas.59.2.484
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发表时间：
1968-01-01
影响因子：
11.1
通讯作者：
THOMPSON, TE
中科院分区：
文献类型：
--
作者：
HOPFER, U;LEHNINGER, AL;THOMPSON, TE
线粒体解偶联剂2，4-二硝基苯酚（DNP）、羰基氰间氯苯腙（Cl-CCP）、羰基氰对三氟甲氧基苯腙（CF-CCP）和双香豆素增加模型磷脂双层膜的比电导。每种解偶联剂在增加跨膜电导方面表现出最佳pH。在CF-CCP存在下测量跨膜电位，跨膜pH梯度为1.0单位，得到54 mv的一致值，与25[度]纯质子电导预期的59 mv的理论值非常一致。它的结论是，解偶联剂通过促进H+或OH-（或两者）跨膜的运输增加了这种模型膜的比电导，并且它们不促进系统中的其他离子，如K+，Cl-，或Tris+的显着运输。这些结果在定性上与Mitchell提出的解偶联剂对线粒体氧化磷酸化作用的假说雅阁。
The mitochondrial uncoupling agents 2,4-dinitrophenol (DNP), carbonylcyanide m-chlorophenyl-hydrazone (Cl-CCP), carbonylcyanide p-trifluoromethoxyphenylhydrazone (CF-CCP), and dicoumarol increased the specific electrical conductance of model phospholipid bilayer membranes. Each uncoupling agent showed a pH optimum in increasing the transmembrane conductance. Measurement of the transmembrane potential in the presence of CF-CCP with a pH gradient of 1.0 unit across the membrane gave consistent values of 54 mv, in good agreement with the theoretical value of 59 mv expected for pure protonic conductance at 25[degree]. It is concluded that the uncoupling agents increase the specific conductance of such model membranes by facilitating transport of H+ or OH- (or both) across the membrane, and that they do not facilitate significant transport of other ions in the system, such as K+, Cl-, or Tris+. These results are qualitatively in accord with the hypothesis proposed by Mitchell for the action of uncoupling agents on oxidative phosphorylation in mitochondria.